{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mishra J"],"funding":["Veterans Administration"],"pagination":["E1052"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6770067"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(9)"],"pubmed_abstract":["Regulation of mitochondrial free Ca2+ is critically important for cellular homeostasis. An increase in mitochondrial matrix free Ca2+ concentration ([Ca2+]m) predisposes mitochondria to opening of the permeability transition pore (mPTP). Opening of the pore can be delayed by cyclosporin A (CsA), possibly by inhibiting cyclophilin D (Cyp D), a key regulator of mPTP. Here, we report on a novel mechanism by which CsA delays mPTP opening by enhanced sequestration of matrix free Ca2+. Cardiac-isolated mitochondria were challenged with repetitive CaCl2 boluses under Na+-free buffer conditions with and without CsA. CsA significantly delayed mPTP opening primarily by promoting matrix Ca2+ sequestration, leading to sustained basal [Ca2+]m levels for an extended period. The preservation of basal [Ca"],"journal":["Cells"],"pubmed_title":["Cyclosporin A Increases Mitochondrial Buffering of Calcium: An Additional Mechanism in Delaying Mitochondrial Permeability Transition Pore Opening."],"pmcid":["PMC6770067"],"funding_grant_id":["BX-002539-01"],"pubmed_authors":["Davani AJ","Natarajan GK","Camara AKS","Mishra J","Kwok WM","Stowe DF"],"additional_accession":[]},"is_claimable":false,"name":"Cyclosporin A Increases Mitochondrial Buffering of Calcium: An Additional Mechanism in Delaying Mitochondrial Permeability Transition Pore Opening.","description":"Regulation of mitochondrial free Ca2+ is critically important for cellular homeostasis. An increase in mitochondrial matrix free Ca2+ concentration ([Ca2+]m) predisposes mitochondria to opening of the permeability transition pore (mPTP). Opening of the pore can be delayed by cyclosporin A (CsA), possibly by inhibiting cyclophilin D (Cyp D), a key regulator of mPTP. Here, we report on a novel mechanism by which CsA delays mPTP opening by enhanced sequestration of matrix free Ca2+. Cardiac-isolated mitochondria were challenged with repetitive CaCl2 boluses under Na+-free buffer conditions with and without CsA. CsA significantly delayed mPTP opening primarily by promoting matrix Ca2+ sequestration, leading to sustained basal [Ca2+]m levels for an extended period. The preservation of basal [Ca","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2025-04-20T00:25:17.126Z","creation":"2019-11-07T08:06:18Z"},"accession":"S-EPMC6770067","cross_references":{"pubmed":["31500337"],"doi":["10.3390/cells8091052"]}}