biostudies-literatureAbeywickrema MEuropean Molecular Biology OrganizationGrantová Agentura České RepublikyAkademie Věd České RepublikyPřírodovědecká Fakulta, Univerzita KarlovaWellcome TrustBiotechnology and Biological Sciences Research Council1024-1040https://www.ebi.ac.uk/biostudies/studies/S-EPMC6771564biostudies-literatureUnknown112(3)Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. Conversely, proliferative cell divisions produce two daughter cells, which look similar but are not identical. To examine in detail differences between the daughter cells of a proliferative division of procyclic T. brucei we used the recently identified constituents of the flagella connector. These segregate asymmetrically during cytokinesis allowing the new-flagellum and the old-flagellum daughters to be distinguished. We discovered that there are distinct morphological differences between the two daughters, with the new-flagellum daughter in particular re-modelling rapidly anMolecular microbiologyNon-equivalence in old- and new-flagellum daughter cells of a proliferative division in Trypanosoma brucei.PMC6771564211075/Z/18/ZInstallation Grant103261/Z/13/Z16-26444YBB/M000532/1104627/Z/14/ZPhD fellowshipJ. E. Purkyne FellowshipVachova HMohr TGull KVarga VAbeywickrema MLai DHVaughan SSunter JDFarr HWheeler RJfalseNon-equivalence in old- and new-flagellum daughter cells of a proliferative division in Trypanosoma brucei.Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. Conversely, proliferative cell divisions produce two daughter cells, which look similar but are not identical. To examine in detail differences between the daughter cells of a proliferative division of procyclic T. brucei we used the recently identified constituents of the flagella connector. These segregate asymmetrically during cytokinesis allowing the new-flagellum and the old-flagellum daughters to be distinguished. We discovered that there are distinct morphological differences between the two daughters, with the new-flagellum daughter in particular re-modelling rapidly an2019-01-01T00:00:00Z2019 Sep2025-04-04T08:26:10.096Z2019-10-11T07:14:04ZS-EPMC67715643128658310.1111/mmi.14345