{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Minhas R"],"funding":["Fundacja na rzecz Nauki Polskiej","Narodowe Centrum Nauki"],"pagination":["837-849"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6771876"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["248(9)"],"pubmed_abstract":["<h4>Background</h4>Identifying enhancers and deciphering their putative roles represent a major step to better understand the mechanism of metazoan gene regulation, development, and the role of regulatory elements in disease. Comparative genomics and transgenic assays have been used with some success to identify critical regions that are involved in regulating the spatiotemporal expression of genes during embryogenesis.<h4>Results</h4>We identified two novel tetrapod-teleost conserved noncoding elements within the vicinity of the zic3 and zic6 loci in the zebrafish genome and demonstrated their ability to drive tissue-specific expression in a transgenic zebrafish assay. The syntenic analysis and robust green fluorescent expression in the developing habenula in the stable transgenic line we"],"journal":["Developmental dynamics : an official publication of the American Association of Anatomists"],"pubmed_title":["A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression."],"pmcid":["PMC6771876"],"funding_grant_id":["POIR.04.04.00‐00‐1AF0/16‐00/","2015/19/P/NZ3/03613","2016/21/B/NZ3/00354"],"pubmed_authors":["Paterek A","Lapinski M","Winata CL","Bazala M","Korzh V","Minhas R"],"additional_accession":[]},"is_claimable":false,"name":"A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression.","description":"<h4>Background</h4>Identifying enhancers and deciphering their putative roles represent a major step to better understand the mechanism of metazoan gene regulation, development, and the role of regulatory elements in disease. Comparative genomics and transgenic assays have been used with some success to identify critical regions that are involved in regulating the spatiotemporal expression of genes during embryogenesis.<h4>Results</h4>We identified two novel tetrapod-teleost conserved noncoding elements within the vicinity of the zic3 and zic6 loci in the zebrafish genome and demonstrated their ability to drive tissue-specific expression in a transgenic zebrafish assay. The syntenic analysis and robust green fluorescent expression in the developing habenula in the stable transgenic line we","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2025-04-26T06:48:05.321Z","creation":"2019-10-16T07:05:48Z"},"accession":"S-EPMC6771876","cross_references":{"pubmed":["31194899"],"doi":["10.1002/dvdy.69"]}}