{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ault TB"],"funding":["Angus Foundation for providing"],"pagination":["4305-4313"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6776284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(10)"],"pubmed_abstract":["The current study characterized the taxonomic composition of the uterine and vaginal bacterial communities during estrous synchronization up to timed artificial insemination (TAI). Postpartum beef cows (n = 68) were subjected to pre-synchronization step 21 d prior to TAI (day -21), followed by an industry standard 7 Day Co-Synch on day -9 and TAI on day 0. Uterine and vaginal flushes were collected on days -21, -9, and -2 of the protocol and pH was immediately recorded. Pregnancy was determined by transrectal ultrasound on day 30. Bacterial DNA was extracted and sequenced targeting the V1 to V3 hypervariable regions of the 16S rRNA bacterial gene. Results indicated 34 different phyla including 792 different genera present between the uterus and vagina. Many differences in the relative abun"],"journal":["Journal of animal science"],"pubmed_title":["Bacterial taxonomic composition of the postpartum cow uterus and vagina prior to artificial insemination1."],"pmcid":["PMC6776284"],"funding_grant_id":["A16-1356-001"],"pubmed_authors":["Pohler KG","Ault TB","Myer PR","Dantas FG","Franco GA","Edwards JL","Smith TPL","Clemmons BA","Reese ST"],"additional_accession":[]},"is_claimable":false,"name":"Bacterial taxonomic composition of the postpartum cow uterus and vagina prior to artificial insemination1.","description":"The current study characterized the taxonomic composition of the uterine and vaginal bacterial communities during estrous synchronization up to timed artificial insemination (TAI). Postpartum beef cows (n = 68) were subjected to pre-synchronization step 21 d prior to TAI (day -21), followed by an industry standard 7 Day Co-Synch on day -9 and TAI on day 0. Uterine and vaginal flushes were collected on days -21, -9, and -2 of the protocol and pH was immediately recorded. Pregnancy was determined by transrectal ultrasound on day 30. Bacterial DNA was extracted and sequenced targeting the V1 to V3 hypervariable regions of the 16S rRNA bacterial gene. Results indicated 34 different phyla including 792 different genera present between the uterus and vagina. Many differences in the relative abun","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2025-04-19T04:31:22.608Z","creation":"2020-10-08T07:22:41Z"},"accession":"S-EPMC6776284","cross_references":{"pubmed":["31251804"],"doi":["10.1093/jas/skz212"]}}