{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Nakamura Y"],"funding":["Japan Science and Technology Agency"],"pubmed_abstract":["The exogenous Ca2+ chelator EGTA (ethylene glycol tetraacetic acid) has been widely used to probe the coupling distance between Ca2+ channels and vesicular Ca2+ sensors for neurotransmitter release. Because of its slow forward rate for binding, EGTA is thought to not capture calcium ions in very proximity to a channel, whereas it does capture calcium ions at the remote distance. However, in this study, our reaction diffusion simulations (RDSs) of Ca2+ combined with a release calculation using vesicular sensor models indicate that a high concentration of EGTA decreases Ca2+ and vesicular release in the nanodomain of single channels. We found that a key determinant of the effect of EGTA on neurotransmitter release is the saturation of the vesicular sensor. When the sensor is saturated, the r"],"journal":["Frontiers in synaptic neuroscience"],"pagination":["26"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6779814"],"repository":["biostudies-literature"],"pubmed_title":["EGTA Can Inhibit Vesicular Release in the Nanodomain of Single Ca2+ Channels."],"pmcid":["PMC6779814"],"pubmed_authors":["Nakamura Y"],"additional_accession":[]},"is_claimable":false,"name":"EGTA Can Inhibit Vesicular Release in the Nanodomain of Single Ca2+ Channels.","description":"The exogenous Ca2+ chelator EGTA (ethylene glycol tetraacetic acid) has been widely used to probe the coupling distance between Ca2+ channels and vesicular Ca2+ sensors for neurotransmitter release. Because of its slow forward rate for binding, EGTA is thought to not capture calcium ions in very proximity to a channel, whereas it does capture calcium ions at the remote distance. However, in this study, our reaction diffusion simulations (RDSs) of Ca2+ combined with a release calculation using vesicular sensor models indicate that a high concentration of EGTA decreases Ca2+ and vesicular release in the nanodomain of single channels. We found that a key determinant of the effect of EGTA on neurotransmitter release is the saturation of the vesicular sensor. When the sensor is saturated, the r","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-18T17:11:06.643Z","creation":"2019-10-30T08:17:53Z"},"accession":"S-EPMC6779814","cross_references":{"pubmed":["31632263"],"doi":["10.3389/fnsyn.2019.00026"]}}