biostudies-literatureRivera-Chavez JNCCIH NIH HHSNational Cancer InstituteNCI NIH HHS594-597https://www.ebi.ac.uk/biostudies/studies/S-EPMC6785197biostudies-literatureUnknown60(8)A new 8,8'-binaphthopyranone (mycopyranone, <b>1</b>) was isolated from a solid fermentation of <i>Phialemoniopsis</i> sp. (fungal strain MSX61662), and the structure was elucidated via analysis of the NMR and HRESIMS data. The axial chirality of <b>1</b> was determined to be <i>M</i> by ECD. The central chirality at C-4/C-4' was assigned through a modified Mosher's method, while the absolute configuration at C-3/C-3' was deduced based on analysis of the ³ <i>J</i> _H-3-H-4 values and NOESY correlations. Compound <b>1</b> was evaluated for its antimicrobial properties against <i>Staphylococcus aureus</i> SA1199 and a clinically relevant methicillin-resistant <i>S. aureus</i> strain (MRSA USA300 LAC strain AH1263). Compound <b>1</b> inhibited the growth of both strains Tetrahedron lettersMycopyranone: a 8,8'-binaphthopyranone with potent anti-MRSA activity from the fungus <i>Phialemoniopsis</i> sp.PMC6785197P01 CA125066T32 AT008938Cech NBPearce CJGarcia-Salazar JJRaja HAOberlies NHRivera-Chavez JCaesar LfalseMycopyranone: a 8,8'-binaphthopyranone with potent anti-MRSA activity from the fungus <i>Phialemoniopsis</i> sp.A new 8,8'-binaphthopyranone (mycopyranone, <b>1</b>) was isolated from a solid fermentation of <i>Phialemoniopsis</i> sp. (fungal strain MSX61662), and the structure was elucidated via analysis of the NMR and HRESIMS data. The axial chirality of <b>1</b> was determined to be <i>M</i> by ECD. The central chirality at C-4/C-4' was assigned through a modified Mosher's method, while the absolute configuration at C-3/C-3' was deduced based on analysis of the ³ <i>J</i> _H-3-H-4 values and NOESY correlations. Compound <b>1</b> was evaluated for its antimicrobial properties against <i>Staphylococcus aureus</i> SA1199 and a clinically relevant methicillin-resistant <i>S. aureus</i> strain (MRSA USA300 LAC strain AH1263). Compound <b>1</b> inhibited the growth of both strains 2019-01-01T00:00:00Z2019 Feb2026-04-29T03:43:44.854Z2020-05-22T10:35:48ZS-EPMC67851973159801410.1016/j.tetlet.2019.01.029