{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Keren L"],"funding":["NIBIB NIH HHS","NIA NIH HHS","NIDA NIH HHS","NIAID NIH HHS","NCI NIH HHS","NINDS NIH HHS","National Institutes of Health","NIAMS NIH HHS","NIH HHS","NIGMS NIH HHS","National Science Foundation Graduate Research Fellowship"],"pagination":["eaax5851"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6785247"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(10)"],"pubmed_abstract":["Understanding tissue structure and function requires tools that quantify the expression of multiple proteins while preserving spatial information. Here, we describe MIBI-TOF (multiplexed ion beam imaging by time of flight), an instrument that uses bright ion sources and orthogonal time-of-flight mass spectrometry to image metal-tagged antibodies at subcellular resolution in clinical tissue sections. We demonstrate quantitative, full periodic table coverage across a five-log dynamic range, imaging 36 labeled antibodies simultaneously with histochemical stains and endogenous elements. We image fields of view up to 800 μm × 800 μm at resolutions down to 260 nm with sensitivities approaching single-molecule detection. We leverage these properties to interrogate intrapatient heterogeneity in tu"],"journal":["Science advances"],"pubmed_title":["MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure."],"pmcid":["PMC6785247"],"funding_grant_id":["R01 DA022550","U24 CA224309","DP2 EB024246","U19 AI116484","U19 AI104209","R01 AG057915","DP5 OD019822","P50 NS062684","5-T32-AI07290-33","R01 AG056287","R01 CA183904","R21 AG032494","Grant No. 2017242837","R01 AR067145","K99 GM104148","AI07290"],"pubmed_authors":["Bendall SC","Angelo M","Angoshtari R","Fienberg H","Bosse M","Wang J","Keren L","Thompson S","Kambham N","Nolan G","Greenwald NF","Galli SJ","Vijayaragavan K","Risom T","Marquez D","West R","McCaffrey E","Montine TJ","Kirkwood D"],"additional_accession":[]},"is_claimable":false,"name":"MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure.","description":"Understanding tissue structure and function requires tools that quantify the expression of multiple proteins while preserving spatial information. Here, we describe MIBI-TOF (multiplexed ion beam imaging by time of flight), an instrument that uses bright ion sources and orthogonal time-of-flight mass spectrometry to image metal-tagged antibodies at subcellular resolution in clinical tissue sections. We demonstrate quantitative, full periodic table coverage across a five-log dynamic range, imaging 36 labeled antibodies simultaneously with histochemical stains and endogenous elements. We image fields of view up to 800 μm × 800 μm at resolutions down to 260 nm with sensitivities approaching single-molecule detection. We leverage these properties to interrogate intrapatient heterogeneity in tu","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2025-04-19T17:10:09.177Z","creation":"2019-10-30T08:19:11Z"},"accession":"S-EPMC6785247","cross_references":{"pubmed":["31633026"],"doi":["10.1126/sciadv.aax5851"]}}