biostudies-literatureQuan HNICHD NIH HHSNational Institutes of HealthNIGMS NIH HHS78https://www.ebi.ac.uk/biostudies/studies/S-EPMC6785909biostudies-literatureUnknown17(1)<h4>Background</h4>The oosome is the germline determinant in the wasp Nasonia vitripennis and is homologous to the polar granules of Drosophila. Despite a common evolutionary origin and developmental role, the oosome is morphologically quite distinct from polar granules. It is a solid sphere that migrates within the cytoplasm before budding out and forming pole cells.<h4>Results</h4>To gain an understanding of both the molecular basis of oosome development and the conserved essential features of germ plasm, we quantified and compared transcript levels between embryo fragments that contained the oosome and those that did not. The identity of the differentially localized transcripts indicated that Nasonia uses a distinct set of molecules to carry out conserved germ plasm functions. In additiBMC biologyTranscriptomic and functional analysis of the oosome, a unique form of germ plasm in the wasp Nasonia vitripennis.PMC6785909R03 HD087476R03 HD0785781R01GM1291531R03HD087476R01 GM1291531R03HD078578Arsala DLynch JAQuan HfalseTranscriptomic and functional analysis of the oosome, a unique form of germ plasm in the wasp Nasonia vitripennis.<h4>Background</h4>The oosome is the germline determinant in the wasp Nasonia vitripennis and is homologous to the polar granules of Drosophila. Despite a common evolutionary origin and developmental role, the oosome is morphologically quite distinct from polar granules. It is a solid sphere that migrates within the cytoplasm before budding out and forming pole cells.<h4>Results</h4>To gain an understanding of both the molecular basis of oosome development and the conserved essential features of germ plasm, we quantified and compared transcript levels between embryo fragments that contained the oosome and those that did not. The identity of the differentially localized transcripts indicated that Nasonia uses a distinct set of molecules to carry out conserved germ plasm functions. In additi2019-01-01T00:00:00Z2019 Oct2026-04-08T02:23:30.805Z2019-10-30T08:19:27ZS-EPMC67859093160121310.1186/s12915-019-0696-7