biostudies-literatureWang BNatural Sciences and Engineering Research Council of CanadaInstitute of Gender and HealthAlberta Cancer Foundation2876-2892https://www.ebi.ac.uk/biostudies/studies/S-EPMC6791708biostudies-literatureUnknown18(21)Glioblastoma is the most aggressive brain tumor. Although miR-141 has been demonstrated to primarily function as a tumor suppressor in numerous malignancies, including glioblastoma, the mechanisms involved remain poorly understood. Here, it is shown that miR-141 is downregulated in glioblastoma cell lines and tissues and may exert its biological function via directly targeting <i>myelin transcription factor 1-like</i> (<i>MYT1L</i>). Using two glioblastoma cell lines that differ from each other by the functionality of DNA-dependent protein kinase (DNAPK), a functional involvement of DNAPK in the miR-141 tumor suppression network was observed. In M059K cells with a normal function of DNAPK, the enforced expression of miR-141 attenuated MYT1L expression and suppressed cell proliferation. ConCell cycle (Georgetown, Tex.)The crucial role of DNA-dependent protein kinase and myelin transcription factor 1-like protein in the miR-141 tumor suppressor network.PMC6791708N/A05519Rodriguez-Juarez RKovalchuk OIlnytskyy YYao YWang BLi DKovalchuk IHeyns MBronson RTKovalchuk AMetz GASfalseThe crucial role of DNA-dependent protein kinase and myelin transcription factor 1-like protein in the miR-141 tumor suppressor network.Glioblastoma is the most aggressive brain tumor. Although miR-141 has been demonstrated to primarily function as a tumor suppressor in numerous malignancies, including glioblastoma, the mechanisms involved remain poorly understood. Here, it is shown that miR-141 is downregulated in glioblastoma cell lines and tissues and may exert its biological function via directly targeting <i>myelin transcription factor 1-like</i> (<i>MYT1L</i>). Using two glioblastoma cell lines that differ from each other by the functionality of DNA-dependent protein kinase (DNAPK), a functional involvement of DNAPK in the miR-141 tumor suppression network was observed. In M059K cells with a normal function of DNAPK, the enforced expression of miR-141 attenuated MYT1L expression and suppressed cell proliferation. Con2019-01-01T00:00:00Z2019 Nov2025-04-22T10:36:16.206Z2020-09-18T07:06:55ZS-EPMC67917083152259510.1080/15384101.2019.1652033