{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Woodcock ME"],"funding":["Innovate UK","RCUK | Biotechnology and Biological Sciences Research Council","EC | Horizon 2020","Biotechnology and Biological Sciences Research Council"],"pagination":["20930-20937"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6800374"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["116(42)"],"pubmed_abstract":["In macrolecithal species, cryopreservation of the oocyte and zygote is not possible due to the large size and quantity of lipid deposited within the egg. For birds, this signifies that cryopreserving and regenerating a species from frozen cellular material are currently technically unfeasible. Diploid primordial germ cells (PGCs) are a potential means to freeze down the entire genome and reconstitute an avian species from frozen material. Here, we examine the use of genetically engineered (GE) sterile female layer chicken as surrogate hosts for the transplantation of cryopreserved avian PGCs from rare heritage breeds of chicken. We first amplified PGC numbers in culture before cryopreservation and subsequent transplantation into host GE embryos. We found that all hatched offspring from the"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Reviving rare chicken breeds using genetically engineered sterility in surrogate host birds."],"pmcid":["PMC6800374"],"funding_grant_id":["BB/N019202/1","BB/P0.13732/1","BBS/E/D/30002275","BB/L018063/1","BBS/E/D/10002071","BBS/E/D/20320000","BBS/E/D/20211550","677353","BB/P013759/1","BB/M011895/1"],"pubmed_authors":["Taylor L","Burt DW","Mason AS","McGrew MJ","Gheyas AA","Nandi S","Sherman A","Hawken R","Woodcock ME","Smith J"],"additional_accession":[]},"is_claimable":false,"name":"Reviving rare chicken breeds using genetically engineered sterility in surrogate host birds.","description":"In macrolecithal species, cryopreservation of the oocyte and zygote is not possible due to the large size and quantity of lipid deposited within the egg. For birds, this signifies that cryopreserving and regenerating a species from frozen cellular material are currently technically unfeasible. Diploid primordial germ cells (PGCs) are a potential means to freeze down the entire genome and reconstitute an avian species from frozen material. Here, we examine the use of genetically engineered (GE) sterile female layer chicken as surrogate hosts for the transplantation of cryopreserved avian PGCs from rare heritage breeds of chicken. We first amplified PGC numbers in culture before cryopreservation and subsequent transplantation into host GE embryos. We found that all hatched offspring from the","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2025-04-20T01:59:17.578Z","creation":"2021-02-21T02:52:16Z"},"accession":"S-EPMC6800374","cross_references":{"pubmed":["31575742"],"doi":["10.1073/pnas.1906316116"]}}