{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(6)"],"submitter":["Narimani M"],"pubmed_abstract":["Background
Acute myelocytic leukemia (AML) is a clonal malignancy resulting from the accumulation of genetic abnormalities in the cells. Human baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5), encodes survivin, is one of only a handful of genes that is differentially over-expressed in numerous malignant diseases including AML.Methods
The BIRC5 was silenced permanently in two AML cell lines, HL‑60 and KG-1, via the CRISPR/Cas9n system. After transfection of CRISPR constructs, genomic DNA was extracted and amplified to assess mutation detection. To evaluate BIRC5 gene expression, quantitative real-time PCR was performed. Also, MTT cell viability and Annexin‑V/propidium iodide flowcytometric staining were performed, and the data were analyzed using the Kolmogorov"],"journal":["Iranian biomedical journal"],"pagination":["369-78"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6800533"],"repository":["biostudies-literature"],"pubmed_title":["BIRC5 Gene Disruption via CRISPR/Cas9n Platform Suppress Acute Myelocytic Leukemia Progression"],"pmcid":["PMC6800533"],"pubmed_authors":["Roshani D","Sharifi M","Hejazi SH","Narimani M","Jalili A","Kazemi M","Hakhamaneshi MS"],"additional_accession":[]},"is_claimable":false,"name":"BIRC5 Gene Disruption via CRISPR/Cas9n Platform Suppress Acute Myelocytic Leukemia Progression","description":"Background
Acute myelocytic leukemia (AML) is a clonal malignancy resulting from the accumulation of genetic abnormalities in the cells. Human baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5), encodes survivin, is one of only a handful of genes that is differentially over-expressed in numerous malignant diseases including AML.Methods
The BIRC5 was silenced permanently in two AML cell lines, HL‑60 and KG-1, via the CRISPR/Cas9n system. After transfection of CRISPR constructs, genomic DNA was extracted and amplified to assess mutation detection. To evaluate BIRC5 gene expression, quantitative real-time PCR was performed. Also, MTT cell viability and Annexin‑V/propidium iodide flowcytometric staining were performed, and the data were analyzed using the Kolmogorov","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Nov","modification":"2025-04-18T20:03:38.403Z","creation":"2025-04-07T07:49:21.315Z"},"accession":"S-EPMC6800533","cross_references":{"pubmed":["31104397"]}}