{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tsankov AM"],"funding":["NIDDK NIH HHS","NIAID NIH HHS","NHGRI NIH HHS","NIGMS NIH HHS"],"pagination":["dev174722"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6803377"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["146(19)"],"pubmed_abstract":["Maintenance of pluripotency and specification towards a new cell fate are both dependent on precise interactions between extrinsic signals and transcriptional and epigenetic regulators. Directed methylation of cytosines by the <i>de novo</i> methyltransferases DNMT3A and DNMT3B plays an important role in facilitating proper differentiation, whereas DNMT1 is essential for maintaining global methylation levels in all cell types. Here, we generated single-cell mRNA expression data from wild-type, DNMT3A, DNMT3A/3B and DNMT1 knockout human embryonic stem cells and observed a widespread increase in cellular and transcriptional variability, even with limited changes in global methylation levels in the <i>de novo</i> knockouts. Furthermore, we found unexpected transcriptional repression upon eith"],"journal":["Development (Cambridge, England)"],"pubmed_title":["Loss of DNA methyltransferase activity in primed human ES cells triggers increased cell-cell variability and transcriptional repression."],"pmcid":["PMC6803377"],"funding_grant_id":["DP3 DK111898","U24 AI118672","P01 GM099117","DP2 GM119419","RM1 HG006193"],"pubmed_authors":["Allon SJ","Meissner A","Akopian V","Mead BE","Shalek AK","Charlton J","Arczewska A","Tsankov AM","Wadsworth MH","Mikkelsen TS","Drake RS","Smith ZD"],"additional_accession":[]},"is_claimable":false,"name":"Loss of DNA methyltransferase activity in primed human ES cells triggers increased cell-cell variability and transcriptional repression.","description":"Maintenance of pluripotency and specification towards a new cell fate are both dependent on precise interactions between extrinsic signals and transcriptional and epigenetic regulators. Directed methylation of cytosines by the <i>de novo</i> methyltransferases DNMT3A and DNMT3B plays an important role in facilitating proper differentiation, whereas DNMT1 is essential for maintaining global methylation levels in all cell types. Here, we generated single-cell mRNA expression data from wild-type, DNMT3A, DNMT3A/3B and DNMT1 knockout human embryonic stem cells and observed a widespread increase in cellular and transcriptional variability, even with limited changes in global methylation levels in the <i>de novo</i> knockouts. Furthermore, we found unexpected transcriptional repression upon eith","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2026-05-04T23:39:55.727Z","creation":"2020-10-08T07:28:20Z"},"accession":"S-EPMC6803377","cross_references":{"pubmed":["31515224"],"doi":["10.1242/dev.174722"]}}