biostudies-literatureUnknown6Beshir WFIn developing apple fruit, metabolic compartmentation is poorly understood due to the lack of experimental data. Distinguishing subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the technique. The work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit, covering the major events occurring during fruit development (cell division, cell expansion, and maturation). Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments (cytosol, plastids, and vacHorticulture research98https://www.ebi.ac.uk/biostudies/studies/S-EPMC6804870biostudies-literatureNon-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development.PMC6804870Tohge TNicolai BMFernie ARBeshir WFHertog MLATMWatanabe MHoefgen RfalseNon-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development.In developing apple fruit, metabolic compartmentation is poorly understood due to the lack of experimental data. Distinguishing subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the technique. The work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit, covering the major events occurring during fruit development (cell division, cell expansion, and maturation). Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments (cytosol, plastids, and vac2019-01-01T00:00:00Z20192026-05-07T03:47:55.843Z2019-11-07T08:05:23ZS-EPMC68048703166695910.1038/s41438-019-0178-7