biostudies-literatureUnknown19Yu GJ<h4>Background</h4>LncRNAs may exert a regulatory effect in tumorigenesis. Although the expression of lncRNA <i>HOTAIR</i> has been confirmed to be notably elevated in the tissues of CSCC, its biological mechanism in CSCC is still unknown.<h4>Methods</h4><i>HOTAIR</i> expression level in CSCC cell lines was monitored via qRT-PCR. Then CCK-8 assay, Transwell assay and EdU assay were adopted to detect cell migration and proliferation. Meanwhile, through bioinformatics analysis and luciferase reporter gene detection, a new target of <i>HOTAIR</i> was identified. Additionally, Western blotting and RIP analysis were adopted to discuss the possible mechanism.<h4>Results</h4><i>HOTAIR</i> expression in CSCC cell lines exhibited an obvious elevation. Cell function analysis revealed that <i>HOTAIR<Cancer cell international270https://www.ebi.ac.uk/biostudies/studies/S-EPMC6805682biostudies-literatureLong non-coding RNA HOTAIR functions as a competitive endogenous RNA to regulate PRAF2 expression by sponging miR-326 in cutaneous squamous cell carcinoma.PMC6805682Zhang DWYu GJSun YZhang PfalseLong non-coding RNA HOTAIR functions as a competitive endogenous RNA to regulate PRAF2 expression by sponging miR-326 in cutaneous squamous cell carcinoma.<h4>Background</h4>LncRNAs may exert a regulatory effect in tumorigenesis. Although the expression of lncRNA <i>HOTAIR</i> has been confirmed to be notably elevated in the tissues of CSCC, its biological mechanism in CSCC is still unknown.<h4>Methods</h4><i>HOTAIR</i> expression level in CSCC cell lines was monitored via qRT-PCR. Then CCK-8 assay, Transwell assay and EdU assay were adopted to detect cell migration and proliferation. Meanwhile, through bioinformatics analysis and luciferase reporter gene detection, a new target of <i>HOTAIR</i> was identified. Additionally, Western blotting and RIP analysis were adopted to discuss the possible mechanism.<h4>Results</h4><i>HOTAIR</i> expression in CSCC cell lines exhibited an obvious elevation. Cell function analysis revealed that <i>HOTAIR<2019-01-01T00:00:00Z20192025-06-01T01:14:48.001Z2025-06-01T01:14:48.001ZS-EPMC68056823164948710.1186/s12935-019-0992-x