{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Schalbetter SA"],"funding":["European Research Council","Medical Research Council","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["4795"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6805904"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromosome organisation is linked to genomic sequence. As cells enter meiosis, we observe that strong cohesin-dependent grid-like Hi-C interaction patterns emerge, reminiscent of mammalian interphase organisation, but with distinct regulation. Meiotic patterns agree with simulations of loop extrusion with growth limited by barriers, in which a heterogeneous population of expanding loops develop along the chromosome. Importantly, CTCF, the factor that imposes similar features in mammalian interphase, is absent in S. cerevisiae, suggesting alternat"],"journal":["Nature communications"],"pubmed_title":["Principles of meiotic chromosome assembly revealed in S. cerevisiae."],"pmcid":["PMC6805904"],"funding_grant_id":["311336","BB/M010279/1","BB/J018554/1","G0800005","200843/Z/16/Z","BB/S001425/1"],"pubmed_authors":["Baxter J","Fudenberg G","Pollard KS","Neale MJ","Schalbetter SA"],"additional_accession":[]},"is_claimable":false,"name":"Principles of meiotic chromosome assembly revealed in S. cerevisiae.","description":"During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromosome organisation is linked to genomic sequence. As cells enter meiosis, we observe that strong cohesin-dependent grid-like Hi-C interaction patterns emerge, reminiscent of mammalian interphase organisation, but with distinct regulation. Meiotic patterns agree with simulations of loop extrusion with growth limited by barriers, in which a heterogeneous population of expanding loops develop along the chromosome. Importantly, CTCF, the factor that imposes similar features in mammalian interphase, is absent in S. cerevisiae, suggesting alternat","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2026-05-07T03:48:53.148Z","creation":"2019-11-05T08:10:00Z"},"accession":"S-EPMC6805904","cross_references":{"pubmed":["31641121"],"doi":["10.1038/s41467-019-12629-0"]}}