biostudies-literatureZhang JNatural Sciences and Engineering Research Council of CanadaFundamental Research Funds for the Central UniversitiesNational Key R&D Program of China111 ProjectNational Natural Science Foundation of ChinaState Key Laboratory of Bioreactor Engineering173-179https://www.ebi.ac.uk/biostudies/studies/S-EPMC6807035biostudies-literatureUnknown4(4)Microbes serve as the most important resource for drug discovery. During our screening for bioactive compounds from our natural products library, a pathogenic fungus, Microdochium majus strain 99049, from wheat was selected for further investigation. A new alkaloid named brocaeloid D (1), together with six previously characterized compounds (2-7) were identified. Compound 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide substructure. All the structures of these newly isolated compounds were determined by different means in spectroscopic experiments. The absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound 2. The bioactivities of these identified compounds wSynthetic and systems biotechnologyBrocaeloid D, a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049.PMC68070352187703831430002313201039112017YFE010820081573341B180223172010390122221818014Ma RJiang LZhang JHsiang TZhu GZhang LLi LLiu XHou CHan JWang ZSong ZKarthik LfalseBrocaeloid D, a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049.Microbes serve as the most important resource for drug discovery. During our screening for bioactive compounds from our natural products library, a pathogenic fungus, Microdochium majus strain 99049, from wheat was selected for further investigation. A new alkaloid named brocaeloid D (1), together with six previously characterized compounds (2-7) were identified. Compound 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide substructure. All the structures of these newly isolated compounds were determined by different means in spectroscopic experiments. The absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound 2. The bioactivities of these identified compounds w2019-01-01T00:00:00Z2019 Dec2026-05-04T17:13:21.074Z2019-11-07T08:05:38ZS-EPMC68070353166736710.1016/j.synbio.2019.09.001