biostudies-literaturePostigo-Fernandez JNCATS NIH HHSNIDDK NIH HHSNCRR NIH HHSNIH HHS2040-2051https://www.ebi.ac.uk/biostudies/studies/S-EPMC6812633biostudies-literatureUnknown62(11)<h4>Aims/hypothesis</h4>Tolerance induction in lymph nodes can be mediated by both haematopoietic cells (e.g. specific dendritic cells subsets) and by non-haematopoietic cells (e.g. lymph node stromal cells [LNSCs]) when they present peripheral tissue antigens to autoreactive T cells. LNSCs normally regulate T cell trafficking and survival and help to maintain peripheral tolerance by exerting immunosuppressive effects. However, whether autoimmunity can be associated with defective tolerogenic functions of LNSCs is unknown and studies aimed at characterising LNSCs in humans are lacking. We hypothesised that dysregulated T cell responses in pancreatic lymph nodes (PLNs) from donors with type 1 diabetes and from NOD mice may be associated with altered LNSC function.<h4>Methods</h4>We analysedDiabetologiaPhenotypic alterations in pancreatic lymph node stromal cells from human donors with type 1 diabetes and NOD mice.PMC6812633S10 OD020056S10 RR027050UL1 TR000040R01 DK064819P30 DK063608Postigo-Fernandez JFarber DLCreusot RJfalsePhenotypic alterations in pancreatic lymph node stromal cells from human donors with type 1 diabetes and NOD mice.<h4>Aims/hypothesis</h4>Tolerance induction in lymph nodes can be mediated by both haematopoietic cells (e.g. specific dendritic cells subsets) and by non-haematopoietic cells (e.g. lymph node stromal cells [LNSCs]) when they present peripheral tissue antigens to autoreactive T cells. LNSCs normally regulate T cell trafficking and survival and help to maintain peripheral tolerance by exerting immunosuppressive effects. However, whether autoimmunity can be associated with defective tolerogenic functions of LNSCs is unknown and studies aimed at characterising LNSCs in humans are lacking. We hypothesised that dysregulated T cell responses in pancreatic lymph nodes (PLNs) from donors with type 1 diabetes and from NOD mice may be associated with altered LNSC function.<h4>Methods</h4>We analysed2019-01-01T00:00:00Z2019 Nov2026-04-30T05:21:42.043Z2020-11-03T08:04:04ZS-EPMC68126333148685410.1007/s00125-019-04984-w