biostudies-literaturePoncin KAgence Nationale de la RechercheFonds De La Recherche Scientifique - FNRS4847https://www.ebi.ac.uk/biostudies/studies/S-EPMC6813329biostudies-literatureUnknown10(1)It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.Nature communicationsOccurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.PMC6813329PDR T.0060.15ANR-JCJC-2011Jimmidi RDe Bolle XWillemart KMuraille ERoba ABiondi EGPoncin KFrancis NPotemberg GFioravanti AZeippen NVincent SPMachelart AfalseOccurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.2019-01-01T00:00:00Z2019 Oct2024-12-04T08:22:14.214Z2019-11-07T08:07:02ZS-EPMC68133293164924810.1038/s41467-019-12516-8