{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gu C"],"funding":["HHS | National Institutes of Health","Vetenskapsrådet","European Research Council","Knut och Alice Wallenbergs Stiftelse"],"pagination":["21409-21415"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6815131"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["116(43)"],"pubmed_abstract":["Electrochemical techniques with disk and nano-tip electrodes, together with calcium imaging, were used to examine the effect of short-interval repetitive stimuli on both exocytosis and vesicular content in a model cell line. We show that the number of events decreases markedly with repeated stimuli suggesting a depletion of exocytosis machinery. However, repetitive stimuli induce a more stable fusion pore, leading to an increased amount of neurotransmitter release. In contrast, the total neurotransmitter content inside the vesicles decreases after repetitive stimuli, resulting in a higher average release fraction from each event. We suggest a possible mechanism regarding a link between activity-induced plasticity and fraction of release."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released."],"pmcid":["PMC6815131"],"funding_grant_id":["2017-04366","no number","Scholar","R01GM113746","741903"],"pubmed_authors":["Larsson A","Gu C","Ewing AG"],"additional_accession":[]},"is_claimable":false,"name":"Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released.","description":"Electrochemical techniques with disk and nano-tip electrodes, together with calcium imaging, were used to examine the effect of short-interval repetitive stimuli on both exocytosis and vesicular content in a model cell line. We show that the number of events decreases markedly with repeated stimuli suggesting a depletion of exocytosis machinery. However, repetitive stimuli induce a more stable fusion pore, leading to an increased amount of neurotransmitter release. In contrast, the total neurotransmitter content inside the vesicles decreases after repetitive stimuli, resulting in a higher average release fraction from each event. We suggest a possible mechanism regarding a link between activity-induced plasticity and fraction of release.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2024-02-15T22:03:00.797Z","creation":"2021-02-20T12:14:03Z"},"accession":"S-EPMC6815131","cross_references":{"pubmed":["31570594"],"doi":["10.1073/pnas.1910859116"]}}