{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Akerberg BN"],"funding":["NICHD NIH HHS","NHLBI NIH HHS","NHGRI NIH HHS","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute"],"pagination":["4907"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6817842"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Mapping the chromatin occupancy of transcription factors (TFs) is a key step in deciphering developmental transcriptional programs. Here we use biotinylated knockin alleles of seven key cardiac TFs (GATA4, NKX2-5, MEF2A, MEF2C, SRF, TBX5, TEAD1) to sensitively and reproducibly map their genome-wide occupancy in the fetal and adult mouse heart. These maps show that TF occupancy is dynamic between developmental stages and that multiple TFs often collaboratively occupy the same chromatin region through indirect cooperativity. Multi-TF regions exhibit features of functional regulatory elements, including evolutionary conservation, chromatin accessibility, and activity in transcriptional enhancer assays. H3K27ac, a feature of many enhancers, incompletely overlaps multi-TF regions, and multi-TF "],"journal":["Nature communications"],"pubmed_title":["A reference map of murine cardiac transcription factor chromatin occupancy identifies dynamic and conserved enhancers."],"pmcid":["PMC6817842"],"funding_grant_id":["R01 HL126509","R01 HD089275","2UM1 HL098166","R01 HL146634","R01 HL132164","U01 HL131003","K99 HL143194","UM1 HL098166","R01 HL109605","R01 HG009663","U01 HL098188","T32 HL007572"],"pubmed_authors":["Ma Q","Liu J","VanDusen NJ","Zhou P","Pu WT","Akerberg BN","Yuan GC","Li K","Wen T","Conlon FL","Dong R","Gu F","Dong K","Zhang X","Zhou B","Zhang B","Wasson L","Sethi I","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"A reference map of murine cardiac transcription factor chromatin occupancy identifies dynamic and conserved enhancers.","description":"Mapping the chromatin occupancy of transcription factors (TFs) is a key step in deciphering developmental transcriptional programs. Here we use biotinylated knockin alleles of seven key cardiac TFs (GATA4, NKX2-5, MEF2A, MEF2C, SRF, TBX5, TEAD1) to sensitively and reproducibly map their genome-wide occupancy in the fetal and adult mouse heart. These maps show that TF occupancy is dynamic between developmental stages and that multiple TFs often collaboratively occupy the same chromatin region through indirect cooperativity. Multi-TF regions exhibit features of functional regulatory elements, including evolutionary conservation, chromatin accessibility, and activity in transcriptional enhancer assays. H3K27ac, a feature of many enhancers, incompletely overlaps multi-TF regions, and multi-TF ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2025-04-19T17:10:43.996Z","creation":"2019-11-07T08:08:21Z"},"accession":"S-EPMC6817842","cross_references":{"pubmed":["31659164"],"doi":["10.1038/s41467-019-12812-3"]}}