biostudies-literatureDrev DOesterreichische NationalbankAustrian Science Fund FWFE1421https://www.ebi.ac.uk/biostudies/studies/S-EPMC6827058biostudies-literatureUnknown11(10)Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein modulating cell-matrix interactions and was found up-regulated in tumor stroma. To explore the effect of high stromal SPARC on colorectal cancer (CRC) cell behavior and clinical outcome, this study determined SPARC expression in patients suffering from stage II and III CRC using a publicly available mRNA data set and immunohistochemistry of tissue microarray sections. Moreover, in vitro co-culture models using CRC cell lines together with colon-associated fibroblasts were established to determine the effect of fibroblast-derived SPARC on cancer cells. In 466 patient samples, high SPARC mRNA was associated with a shorter disease-free survival. In 99 patients of the tissue microarray cohort, high stromal SPARC inCancersImpact of Fibroblast-Derived SPARC on Invasiveness of Colorectal Cancer Cells.PMC6827058P2621815655Gruber ESKenner LThalhammer SStift ABergmann MHarpain FReti ADrev DMarian BBeer AKlimpfinger MfalseImpact of Fibroblast-Derived SPARC on Invasiveness of Colorectal Cancer Cells.Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein modulating cell-matrix interactions and was found up-regulated in tumor stroma. To explore the effect of high stromal SPARC on colorectal cancer (CRC) cell behavior and clinical outcome, this study determined SPARC expression in patients suffering from stage II and III CRC using a publicly available mRNA data set and immunohistochemistry of tissue microarray sections. Moreover, in vitro co-culture models using CRC cell lines together with colon-associated fibroblasts were established to determine the effect of fibroblast-derived SPARC on cancer cells. In 466 patient samples, high SPARC mRNA was associated with a shorter disease-free survival. In 99 patients of the tissue microarray cohort, high stromal SPARC in2019-01-01T00:00:00Z2019 Sep2025-04-18T17:12:01.996Z2020-05-21T19:02:09ZS-EPMC68270583155420810.3390/cancers11101421