biostudies-literatureUnknown20(20)Chen JNon-alcoholic fatty liver disease (NAFLD) can progress from steatosis to non-alcoholic steatohepatitis (NASH) characterized by liver inflammation, possibly leading to cirrhosis and hepatocellular carcinoma (HCC). Mice with impaired macrophage activation, when fed a high-fat diet, develop severe NASH. Evidence is mounting that Kupffer cells are implicated. However, it is unknown whether the resident CD68+ or bone marrow-derived CD11b+ Kupffer cells are involved. Characterization of the FSP1cre-Pparb/d-/- mouse liver revealed that FSP1 is expressed in CD11b+ Kupffer cells. Although these cells only constitute a minute fraction of the liver cell population, Pparb/d deletion in these cells led to remarkable hepatic phenotypic changes. We report that a higher lipid content was present in postnaInternational journal of molecular sciencesE5115https://www.ebi.ac.uk/biostudies/studies/S-EPMC6830345biostudies-literatureThe Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD.PMC6830345Wahli WTan NSChen JSng MKZhuang YfalseThe Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD.Non-alcoholic fatty liver disease (NAFLD) can progress from steatosis to non-alcoholic steatohepatitis (NASH) characterized by liver inflammation, possibly leading to cirrhosis and hepatocellular carcinoma (HCC). Mice with impaired macrophage activation, when fed a high-fat diet, develop severe NASH. Evidence is mounting that Kupffer cells are implicated. However, it is unknown whether the resident CD68+ or bone marrow-derived CD11b+ Kupffer cells are involved. Characterization of the FSP1cre-Pparb/d-/- mouse liver revealed that FSP1 is expressed in CD11b+ Kupffer cells. Although these cells only constitute a minute fraction of the liver cell population, Pparb/d deletion in these cells led to remarkable hepatic phenotypic changes. We report that a higher lipid content was present in postna2019-01-01T00:00:00Z2019 Oct2025-04-18T17:13:53.572Z2020-05-21T19:03:42ZS-EPMC68303453161897610.3390/ijms20205115