biostudies-literatureUnknown59(4)Sujitha SThe proportion of people suffering from cardiovascular diseases has risen by 34% in the last 15 years in India. Cardiomyopathy is among the many forms of CVD s present. Infection of heart muscles is the suspected etiological agent for the same. Oral pathogens gaining entry into the bloodstream are responsible for such infections. <i>Streptococcus mutans</i> is an oral pathogen with implications in cardiovascular diseases. Previous studies have shown certain strains of <i>S. mutans</i> are found predominantly within atherosclerotic plaques and extirpated valves. To decipher the genetic differences responsible for endothelial cell invasion, we have sequenced the genome of <i>Streptococcus mutans</i> B14. Pan-genome analysis, search for adhesion proteins through a special algorithm, and protein-protein interactions search through HPIDB have been done. Pan-genome analysis of 187 whole genomes, assemblies revealed 6965 genes in total and 918 genes forming the core gene cluster. Adhesion to the endothelial cell is a critical virulence factor distinguishing virulent and non-virulent strains. Overall, 4% of the total proteins in <i>S. mutans</i> B14 were categorized as adhesion proteins. Protein-protein interaction between putative adhesion proteins and Human extracellular matrix components was predicted, revealing novel interactions. A conserved gene catalyzing the synthesis of branched-chain amino acids in <i>S. mutans</i> B14 shows possible interaction with isoforms of cathepsin protein of the ECM. This genome sequence analysis indicates towards other proteins in the <i>S. mutans</i> genome, which might have a specific role to play in host cell interaction.Indian journal of microbiology451-459https://www.ebi.ac.uk/biostudies/studies/S-EPMC6842392biostudies-literatureGenome Investigation of a Cariogenic Pathogen with Implications in Cardiovascular Diseases.PMC6842392Sujitha SKarthikeyan RRajendhran JSankarasubramanian JVishnu USGunasekaran PfalseGenome Investigation of a Cariogenic Pathogen with Implications in Cardiovascular Diseases.The proportion of people suffering from cardiovascular diseases has risen by 34% in the last 15 years in India. Cardiomyopathy is among the many forms of CVD s present. Infection of heart muscles is the suspected etiological agent for the same. Oral pathogens gaining entry into the bloodstream are responsible for such infections. <i>Streptococcus mutans</i> is an oral pathogen with implications in cardiovascular diseases. Previous studies have shown certain strains of <i>S. mutans</i> are found predominantly within atherosclerotic plaques and extirpated valves. To decipher the genetic differences responsible for endothelial cell invasion, we have sequenced the genome of <i>Streptococcus mutans</i> B14. Pan-genome analysis, search for adhesion proteins through a special algorithm, and protein-protein interactions search through HPIDB have been done. Pan-genome analysis of 187 whole genomes, assemblies revealed 6965 genes in total and 918 genes forming the core gene cluster. Adhesion to the endothelial cell is a critical virulence factor distinguishing virulent and non-virulent strains. Overall, 4% of the total proteins in <i>S. mutans</i> B14 were categorized as adhesion proteins. Protein-protein interaction between putative adhesion proteins and Human extracellular matrix components was predicted, revealing novel interactions. A conserved gene catalyzing the synthesis of branched-chain amino acids in <i>S. mutans</i> B14 shows possible interaction with isoforms of cathepsin protein of the ECM. This genome sequence analysis indicates towards other proteins in the <i>S. mutans</i> genome, which might have a specific role to play in host cell interaction.2019-01-01T00:00:00Z2019 Dec2026-05-03T20:31:31.556Z2021-02-20T02:48:13ZS-EPMC68423923176250810.1007/s12088-019-00823-z