{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(10)"],"submitter":["Borisek J"],"pubmed_abstract":["The spliceosome accurately promotes precursor messenger-RNA splicing by recognizing specific noncoding intronic tracts including the branch point sequence (BPS) and the 3'-splice-site (3'SS). Mutations of Hsh155 (yeast)/SF3B1 (human), which is a protein of the SF3b factor involved in BPS recognition and induces altered BPS binding and 3'SS selection, lead to mis-spliced mRNA transcripts. Although these mutations recur in hematologic malignancies, the mechanism by which they change gene expression remains unclear. In this study, multi-microsecond-long molecular-dynamics simulations of eighth distinct ∼700,000 atom models of the spliceosome Bact complex, and gene sequencing of SF3B1, disclose that these carcinogenic isoforms destabilize intron binding and/or affect the functional dynamics of"],"journal":["Biomolecules"],"pagination":["E633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6843770"],"repository":["biostudies-literature"],"pubmed_title":["Disclosing the Impact of Carcinogenic SF3b Mutations on Pre-mRNA Recognition Via All-Atom Simulations."],"pmcid":["PMC6843770"],"pubmed_authors":["Borisek J","Galli A","Molteni E","Magistrato A","Saltalamacchia A","Palermo G","Malcovati L"],"additional_accession":[]},"is_claimable":false,"name":"Disclosing the Impact of Carcinogenic SF3b Mutations on Pre-mRNA Recognition Via All-Atom Simulations.","description":"The spliceosome accurately promotes precursor messenger-RNA splicing by recognizing specific noncoding intronic tracts including the branch point sequence (BPS) and the 3'-splice-site (3'SS). Mutations of Hsh155 (yeast)/SF3B1 (human), which is a protein of the SF3b factor involved in BPS recognition and induces altered BPS binding and 3'SS selection, lead to mis-spliced mRNA transcripts. Although these mutations recur in hematologic malignancies, the mechanism by which they change gene expression remains unclear. In this study, multi-microsecond-long molecular-dynamics simulations of eighth distinct ∼700,000 atom models of the spliceosome Bact complex, and gene sequencing of SF3B1, disclose that these carcinogenic isoforms destabilize intron binding and/or affect the functional dynamics of","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2026-05-03T20:48:24.062Z","creation":"2020-05-21T20:01:45Z"},"accession":"S-EPMC6843770","cross_references":{"pubmed":["31640290"],"doi":["10.3390/biom9100633"]}}