biostudies-literatureUnknown16(12)Ma LThe detailed pathogenesis of endometriosis remains largely unclear despite decades of research. Recent studies have demonstrated that miRNAs plays an important role in endometriosis. The expression of miR-142-3p was decreased in ectopic endometrial tissues, while KLF9 and VEGFA expression levels were increased. Overexpression of miR-142-3p or knockdown of KLF9 significantly suppressed CRL-7566 cell proliferation and metastasis, induced cell apoptosis, and decreased both cell autophagy and vascularization. Additionally, KLF9 was confirmed to be a direct target of miR-142-3p and to directly bind to the promoter of the <i>VEGFA</i> gene, regulating its expression. Finally, intraperitoneal injection of miR-142-3p lentivirus significantly attenuated ectopic endometriotic lesions <i>in vivo</i>.miR-142-3p directly targeted KLF9, regulated VEGFA expression, and was protective against the growth of ectopic endometriotic lesions. Therefore, the miR-142-3p/KLF9/VEGFA signalling pathway may be a potential target in endometriosis treatment.RNA biology1733-1748https://www.ebi.ac.uk/biostudies/studies/S-EPMC6844572biostudies-literatureMicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy <i>in vitro</i> and <i>in vivo</i>.PMC6844572Li WLi ZMa LChen XAi JfalseMicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy <i>in vitro</i> and <i>in vivo</i>.The detailed pathogenesis of endometriosis remains largely unclear despite decades of research. Recent studies have demonstrated that miRNAs plays an important role in endometriosis. The expression of miR-142-3p was decreased in ectopic endometrial tissues, while KLF9 and VEGFA expression levels were increased. Overexpression of miR-142-3p or knockdown of KLF9 significantly suppressed CRL-7566 cell proliferation and metastasis, induced cell apoptosis, and decreased both cell autophagy and vascularization. Additionally, KLF9 was confirmed to be a direct target of miR-142-3p and to directly bind to the promoter of the <i>VEGFA</i> gene, regulating its expression. Finally, intraperitoneal injection of miR-142-3p lentivirus significantly attenuated ectopic endometriotic lesions <i>in vivo</i>.miR-142-3p directly targeted KLF9, regulated VEGFA expression, and was protective against the growth of ectopic endometriotic lesions. Therefore, the miR-142-3p/KLF9/VEGFA signalling pathway may be a potential target in endometriosis treatment.2019-01-01T00:00:00Z2019 Dec2025-04-04T14:59:35.39Z2020-08-29T07:19:57ZS-EPMC68445723142500410.1080/15476286.2019.1657352