{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["100(2)"],"submitter":["Hu Z"],"funding":["China Scholarship Council","Alexander von Humboldt-Stiftung"],"pubmed_abstract":["To accelerate the isolation of plant protein complexes and study cellular localization and interaction of their components, an improved recombineering protocol is described for simple and fast site-directed modification of plant genes in bacterial artificial chromosomes (BACs). Coding sequences of fluorescent and affinity tags were inserted into genes and transferred together with flanking genomic sequences of desired size by recombination into Agrobacterium plant transformation vectors using three steps of E. coli transformation with PCR-amplified DNA fragments. Application of fast-track recombineering is illustrated by the simultaneous labelling of CYCLIN-DEPENDENT KINASE D (CDKD) and CYCLIN H (CYCH) subunits of kinase module of TFIIH general transcription factor and the CDKD-activating "],"journal":["The Plant journal : for cell and molecular biology"],"pagination":["411-429"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6852550"],"repository":["biostudies-literature"],"pubmed_title":["Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes."],"pmcid":["PMC6852550"],"pubmed_authors":["Ghosh A","Koncz Z","Sarnowski TJ","Bai B","Hu Z","Horvath M","Schaefer S","Liu S","Harzen A","Nakagami H","Hajheidari M","Zundorf S","Stolze SC","Koncz C"],"additional_accession":[]},"is_claimable":false,"name":"Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.","description":"To accelerate the isolation of plant protein complexes and study cellular localization and interaction of their components, an improved recombineering protocol is described for simple and fast site-directed modification of plant genes in bacterial artificial chromosomes (BACs). Coding sequences of fluorescent and affinity tags were inserted into genes and transferred together with flanking genomic sequences of desired size by recombination into Agrobacterium plant transformation vectors using three steps of E. coli transformation with PCR-amplified DNA fragments. Application of fast-track recombineering is illustrated by the simultaneous labelling of CYCLIN-DEPENDENT KINASE D (CDKD) and CYCLIN H (CYCH) subunits of kinase module of TFIIH general transcription factor and the CDKD-activating ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2026-04-15T15:18:56.952Z","creation":"2020-05-21T19:11:04Z"},"accession":"S-EPMC6852550","cross_references":{"pubmed":["31276249"],"doi":["10.1111/tpj.14450"]}}