{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["36(3)"],"submitter":["Hamashima N"],"pubmed_abstract":["Marchantia polymorpha is a model liverwort for which many molecular biological techniques are now available. We previously developed the S-AgarTrap method for easy genetic transformation of M. polymorpha using spores. In this study, we report production of a T-DNA insertion mutant library (approx. 10,000 lines) for M. polymorpha using the S-AgarTrap method. We further isolate and characterize a gain-of-function mutant that hyper-accumulates the flavonoid riccionidin A. The present study demonstrates that the S-AgarTrap-mediated production of a T-DNA insertion mutant library is a powerful tool for molecular biology in M. polymorpha."],"journal":["Plant biotechnology (Tokyo, Japan)"],"pagination":["201-204"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6854341"],"repository":["biostudies-literature"],"pubmed_title":["A gain-of-function T-DNA insertion mutant of Marchantia polymorpha hyper-accumulates flavonoid riccionidin A."],"pmcid":["PMC6854341"],"pubmed_authors":["Hamashima N","Suzuki T","Kodama Y","Xie X","Hikawa M"],"additional_accession":[]},"is_claimable":false,"name":"A gain-of-function T-DNA insertion mutant of Marchantia polymorpha hyper-accumulates flavonoid riccionidin A.","description":"Marchantia polymorpha is a model liverwort for which many molecular biological techniques are now available. We previously developed the S-AgarTrap method for easy genetic transformation of M. polymorpha using spores. In this study, we report production of a T-DNA insertion mutant library (approx. 10,000 lines) for M. polymorpha using the S-AgarTrap method. We further isolate and characterize a gain-of-function mutant that hyper-accumulates the flavonoid riccionidin A. The present study demonstrates that the S-AgarTrap-mediated production of a T-DNA insertion mutant library is a powerful tool for molecular biology in M. polymorpha.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2025-04-04T14:02:05.786Z","creation":"2020-05-21T19:49:34Z"},"accession":"S-EPMC6854341","cross_references":{"pubmed":["31768123"],"doi":["10.5511/plantbiotechnology.19.0722a"]}}