biostudies-literatureBeurton FWellcome Trust Senior Research FellowshipProteomics French InfrastructureFondation ARCCancer Research UKAgence Nationale de la RechercheLabex GRALWellcome Trust11164-11180https://www.ebi.ac.uk/biostudies/studies/S-EPMC6868398biostudies-literatureUnknown47(21)The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin factors contribute to context dependent effects. Using a proteomics approach, we identified CFP1 associated proteins and an unexpected direct link between Caenorhabditis elegans CFP-1 and an Rpd3/Sin3 small (SIN3S) histone deacetylase complex. Supporting a functional connection, we find that mutants of COMPASS and SIN3 complex components genetically interact and have similar phenotypic defects including misregulation of common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 domNucleic acids researchPhysical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans.PMC686839815-CE12-0018-01155265ANR-10-LABX-49-01101863/Z/13/ZC6946/A14492092096101863Coute YCaron MSpichty MDong YHuang NBeurton FAppert ACluet DStempor PBedet CPolveche HHerbette MAhringer JPalladino FChen RAfalsePhysical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans.The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin factors contribute to context dependent effects. Using a proteomics approach, we identified CFP1 associated proteins and an unexpected direct link between Caenorhabditis elegans CFP-1 and an Rpd3/Sin3 small (SIN3S) histone deacetylase complex. Supporting a functional connection, we find that mutants of COMPASS and SIN3 complex components genetically interact and have similar phenotypic defects including misregulation of common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 dom2019-01-01T00:00:00Z2019 Dec2026-04-07T19:17:58.437Z2020-05-21T20:11:55ZS-EPMC68683983160246510.1093/nar/gkz880