biostudies-literatureUnknown9(1)Falker-Gieske CExamples of living sheep-goat hybrids are rare, mainly due to incorrect chromosome pairing, which is thought to be the main cause for species incompatibility. This case represents the first report of a buck-ewe hybrid and the first mammalian hybrid to be analyzed with next generation sequencing. The buck-ewe hybrid had an intermediate karyotype to the parental species, with 57 chromosomes. Analysis of the blood transcriptomes of the hybrid and both parents revealed that gene expression levels differed between the hybrid and its parents. This could be explained in part by age-dependent differences in gene expression. Contribution to the geep transcriptome was larger from the paternal, compared to the maternal, genome. Furthermore, imprinting patterns deviated considerably from what is knownScientific reports17492https://www.ebi.ac.uk/biostudies/studies/S-EPMC6877586biostudies-literatureBlood transcriptome analysis in a buck-ewe hybrid and its parents.PMC6877586Falker-Gieske CKnorr CTetens JfalseBlood transcriptome analysis in a buck-ewe hybrid and its parents.Examples of living sheep-goat hybrids are rare, mainly due to incorrect chromosome pairing, which is thought to be the main cause for species incompatibility. This case represents the first report of a buck-ewe hybrid and the first mammalian hybrid to be analyzed with next generation sequencing. The buck-ewe hybrid had an intermediate karyotype to the parental species, with 57 chromosomes. Analysis of the blood transcriptomes of the hybrid and both parents revealed that gene expression levels differed between the hybrid and its parents. This could be explained in part by age-dependent differences in gene expression. Contribution to the geep transcriptome was larger from the paternal, compared to the maternal, genome. Furthermore, imprinting patterns deviated considerably from what is known2019-01-01T00:00:00Z2019 Nov2026-05-03T20:45:49.544Z2020-05-21T21:03:01ZS-EPMC68775863176794510.1038/s41598-019-53901-z