{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Baers LL"],"funding":["Medical Research Council","Environmental Services Association Education Trust","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["1721-1738"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6878006"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["181(4)"],"pubmed_abstract":["Cyanobacteria are complex prokaryotes, incorporating a Gram-negative cell wall and internal thylakoid membranes (TMs). However, localization of proteins within cyanobacterial cells is poorly understood. Using subcellular fractionation and quantitative proteomics, we produced an extensive subcellular proteome map of an entire cyanobacterial cell, identifying ∼67% of proteins in <i>Synechocystis</i> sp. PCC 6803, ∼1000 more than previous studies. Assigned to six specific subcellular regions were 1,712 proteins. Proteins involved in energy conversion localized to TMs. The majority of transporters, with the exception of a TM-localized copper importer, resided in the plasma membrane (PM). Most metabolic enzymes were soluble, although numerous pathways terminated in the TM (notably those involve"],"journal":["Plant physiology"],"pubmed_title":["Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism."],"pmcid":["PMC6878006"],"funding_grant_id":["BB/N023129/1","BB/S020365/1","110071/Z/15/Z","BB/S507404/1","MC_U105178783","BB/F017464/1","110170/Z/15/Z"],"pubmed_authors":["Lea-Smith DJ","Mills LA","Baers LL","Howe CJ","Gatto L","Lilley KS","Stevens TJ","Breckels LM","Deery MJ"],"additional_accession":[]},"is_claimable":false,"name":"Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism.","description":"Cyanobacteria are complex prokaryotes, incorporating a Gram-negative cell wall and internal thylakoid membranes (TMs). However, localization of proteins within cyanobacterial cells is poorly understood. Using subcellular fractionation and quantitative proteomics, we produced an extensive subcellular proteome map of an entire cyanobacterial cell, identifying ∼67% of proteins in <i>Synechocystis</i> sp. PCC 6803, ∼1000 more than previous studies. Assigned to six specific subcellular regions were 1,712 proteins. Proteins involved in energy conversion localized to TMs. The majority of transporters, with the exception of a TM-localized copper importer, resided in the plasma membrane (PM). Most metabolic enzymes were soluble, although numerous pathways terminated in the TM (notably those involve","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2025-04-04T10:22:26.514Z","creation":"2025-02-19T02:37:56.032Z"},"accession":"S-EPMC6878006","cross_references":{"pubmed":["31578229"],"doi":["10.1104/pp.19.00897"]}}