{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wardell SJT"],"funding":["University of Otago","Manatu Hauora | Health Research Council of New Zealand"],"pagination":["AAC.01619-19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6879238"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(12)"],"pubmed_abstract":["<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes a wide range of acute and chronic infections. An increasing number of isolates have mutations that make them antibiotic resistant, making treatment difficult. To identify resistance-associated mutations we experimentally evolved the antibiotic sensitive strain <i>P. aeruginosa</i> PAO1 to become resistant to three widely used anti-pseudomonal antibiotics, ciprofloxacin, meropenem and tobramycin. Mutants could tolerate up to 2048-fold higher concentrations of antibiotic than strain PAO1. Genome sequences were determined for thirteen mutants for each antibiotic. Each mutant had between 2 and 8 mutations. For each antibiotic at least 8 genes were mutated in multiple mutants, demonstrating the genetic complexity of resistan"],"journal":["Antimicrobial agents and chemotherapy"],"pubmed_title":["A large-scale whole-genome comparison shows that experimental evolution in response to antibiotics predicts changes in naturally evolved clinical <i>Pseudomonas aeruginosa</i>."],"pmcid":["PMC6879238"],"funding_grant_id":["17/372"],"pubmed_authors":["Lamont IL","Patrick WM","Wardell SJT","Martin LW","Rehman A","Winstanley C"],"additional_accession":[]},"is_claimable":false,"name":"A large-scale whole-genome comparison shows that experimental evolution in response to antibiotics predicts changes in naturally evolved clinical <i>Pseudomonas aeruginosa</i>.","description":"<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes a wide range of acute and chronic infections. An increasing number of isolates have mutations that make them antibiotic resistant, making treatment difficult. To identify resistance-associated mutations we experimentally evolved the antibiotic sensitive strain <i>P. aeruginosa</i> PAO1 to become resistant to three widely used anti-pseudomonal antibiotics, ciprofloxacin, meropenem and tobramycin. Mutants could tolerate up to 2048-fold higher concentrations of antibiotic than strain PAO1. Genome sequences were determined for thirteen mutants for each antibiotic. Each mutant had between 2 and 8 mutations. For each antibiotic at least 8 genes were mutated in multiple mutants, demonstrating the genetic complexity of resistan","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2026-05-05T02:20:55.958Z","creation":"2020-05-24T07:08:34Z"},"accession":"S-EPMC6879238","cross_references":{"pubmed":["31570397"],"doi":["10.1128/AAC.01619-19","10.1128/aac.01619-19"]}}