biostudies-literatureTodorovic VJavna Agencija za Raziskovalno Dejavnost RS214https://www.ebi.ac.uk/biostudies/studies/S-EPMC6882348biostudies-literatureUnknown14(1)BACKGROUND:Treatment options for recurrent head and neck tumours in the previously irradiated area are limited, including re-irradiation due to radioresistance of the recurrent tumour and previous dose received by surrounding normal tissues. As an in vitro model to study radioresistance mechanisms, isogenic cells with different radiosensitivity can be used. However, they are not readily available. Therefore, our objective was to establish and characterize radioresistant isogenic human pharyngeal squamous carcinoma cells and to evaluate early radiation response in isogenic parental, radioresistant and radiosensitive cells. METHODS:Radioresistant cells were derived from parental FaDu cells by repeated exposure to ionizing radiation. Radiosensitivity of the established isogenic radioresistantRadiation oncology (London, England)Mechanisms of different response to ionizing irradiation in isogenic head and neck cancer cell lines.PMC6882348J3-7460P3-0003P3-0307Prevc ABrozic ACemazar MTodorovic VSavarin MZakelj MNGroselj BStrojan PSersa GfalseMechanisms of different response to ionizing irradiation in isogenic head and neck cancer cell lines.BACKGROUND:Treatment options for recurrent head and neck tumours in the previously irradiated area are limited, including re-irradiation due to radioresistance of the recurrent tumour and previous dose received by surrounding normal tissues. As an in vitro model to study radioresistance mechanisms, isogenic cells with different radiosensitivity can be used. However, they are not readily available. Therefore, our objective was to establish and characterize radioresistant isogenic human pharyngeal squamous carcinoma cells and to evaluate early radiation response in isogenic parental, radioresistant and radiosensitive cells. METHODS:Radioresistant cells were derived from parental FaDu cells by repeated exposure to ionizing radiation. Radiosensitivity of the established isogenic radioresistant2019-01-01T00:00:00Z2019 Nov2025-04-18T23:39:06.831Z2020-05-21T20:41:10ZS-EPMC68823483177583510.1186/s13014-019-1418-6