biostudies-literatureLorenz DRNCATS NIH HHSNIDA NIH HHSNIAID NIH HHSNHLBI NIH HHSNIMH NIH HHSNational Institutes of HealthNIH HHS59https://www.ebi.ac.uk/biostudies/studies/S-EPMC6883692biostudies-literatureUnknown13(1)<h4>Background</h4>Tobacco smoking induces immunomodulatory and pro-inflammatory effects associated with transcriptome changes in monocytes and other immune cell types. While smoking is prevalent in HIV-infected (HIV+) individuals, few studies have investigated its effects on gene expression in this population. Here, we report whole-transcriptome analyses of 125 peripheral blood monocyte samples from ART-treated HIV+ and uninfected (HIV-) men enrolled in the Multicenter AIDS Cohort Study (MACS) (n = 25 HIV+ smokers, n = 60 HIV+ non-smokers, n = 40 HIV- non-smoking controls). Gene expression profiling was performed using Illumina HumanHT-12 Expression BeadChip microarrays. Differential expression analysis was performed with weighted linear regression models using the R limma package, followHuman genomicsTranscriptomic analysis of monocytes from HIV-positive men on antiretroviral therapy reveals effects of tobacco smoking on interferon and stress response systems associated with depressive symptoms.PMC6883692U01 AI035041MH110259U01 AI035043DA046203U01 AI035042DA40391R01 DA046203U01 HL146201UL1 TR000424R01 MH110259U01 AI035039R01 DA040391Gabuzda DLorenz DRMisra VfalseTranscriptomic analysis of monocytes from HIV-positive men on antiretroviral therapy reveals effects of tobacco smoking on interferon and stress response systems associated with depressive symptoms.<h4>Background</h4>Tobacco smoking induces immunomodulatory and pro-inflammatory effects associated with transcriptome changes in monocytes and other immune cell types. While smoking is prevalent in HIV-infected (HIV+) individuals, few studies have investigated its effects on gene expression in this population. Here, we report whole-transcriptome analyses of 125 peripheral blood monocyte samples from ART-treated HIV+ and uninfected (HIV-) men enrolled in the Multicenter AIDS Cohort Study (MACS) (n = 25 HIV+ smokers, n = 60 HIV+ non-smokers, n = 40 HIV- non-smoking controls). Gene expression profiling was performed using Illumina HumanHT-12 Expression BeadChip microarrays. Differential expression analysis was performed with weighted linear regression models using the R limma package, follow2019-01-01T00:00:00Z2019 Nov2026-05-04T11:34:02.47Z2020-05-21T21:04:15ZS-EPMC68836923177970110.1186/s40246-019-0247-x