biostudies-literatureLiu ILCredo Biomedical Pte. Ltd.National Taiwan University428https://www.ebi.ac.uk/biostudies/studies/S-EPMC6884907biostudies-literatureUnknown15(1)BACKGROUND:Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS:Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30?min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. CONCLUSIONS:This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.BMC veterinary researchA novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs.PMC6884907n.an/aLiu ILChiu CTChi NYChen HWHung MLChang CLfalseA novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs.BACKGROUND:Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS:Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30?min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. CONCLUSIONS:This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.2019-01-01T00:00:00Z2019 Nov2020-10-29T08:06:47Z2020-05-21T20:30:40ZS-EPMC68849073178385010.1186/s12917-019-2181-5