{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ng WH"],"funding":["Kementerian Sains, Teknologi dan Inovasi"],"pagination":["E5519"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6887783"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(22)"],"pubmed_abstract":["Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton's Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower <i>Gata4</i>, <i>Nkx2.5</i>, and <i>Sox2</i> gene expressions, with changes of 2.4, 3767.0, "],"journal":["International journal of molecular sciences"],"pubmed_title":["Human Wharton's Jelly-Derived Mesenchymal Stem Cells Minimally Improve the Growth Kinetics and Cardiomyocyte Differentiation of Aged Murine Cardiac c-kit Cells in In Vitro without Rejuvenating Effect."],"pmcid":["PMC6887783"],"funding_grant_id":["02-01-05-SF0684"],"pubmed_authors":["Ng WH","Yong YK","Lim V","Ramasamy R","Shaharuddin B","Ngalim SH","Tan JJ"],"additional_accession":[]},"is_claimable":false,"name":"Human Wharton's Jelly-Derived Mesenchymal Stem Cells Minimally Improve the Growth Kinetics and Cardiomyocyte Differentiation of Aged Murine Cardiac c-kit Cells in In Vitro without Rejuvenating Effect.","description":"Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton's Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower <i>Gata4</i>, <i>Nkx2.5</i>, and <i>Sox2</i> gene expressions, with changes of 2.4, 3767.0, ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Nov","modification":"2026-04-30T11:06:25.341Z","creation":"2020-05-21T21:20:08Z"},"accession":"S-EPMC6887783","cross_references":{"pubmed":["31698679"],"doi":["10.3390/ijms20225519"]}}