biostudies-literaturePan SPAustrian Science Fund FWF1351https://www.ebi.ac.uk/biostudies/studies/S-EPMC6892967biostudies-literatureUnknown10In order to identify active constituents and to gain some information regarding their mode of action, extracts from leaves of <i>Epipremnum pinnatum</i> were tested for their ability to inhibit inflammatory gene expression in endothelial- and monocyte-like cells (HUVECtert and THP-1, respectively). Bioactivity-guided fractionation using expression of <i>PTGS2</i> (COX-2) mRNA as a readout resulted in the isolation of two C13 megastigmane glycosides, gusanlungionoside C (<b>1</b>) and citroside A (<b>3</b>), and the phenylalcohol glycoside phenylmethyl-2-<i>O</i>-(6-<i>O</i>-rhamnosyl)-ß-D-galactopyranoside (<b>2</b>). Further analysis identified six additional megastigmane glycosides and the aglycones β-damascenone (<b>10</b>), megastigmatrienone (<b>11</b>), 3-hydroxy-β-damascenone (<b>12Frontiers in pharmacologyC13 Megastigmane Derivatives From <i>Epipremnum pinnatum:</i> β-Damascenone Inhibits the Expression of Pro-Inflammatory Cytokines and Leukocyte Adhesion Molecules as Well as NF-κB Signaling.PMC6892967P 27682P 27682-B30Kretschmer NRappai JDirsch VMBauer RBochkov VKunert OPirker THummelbrunner SPan SPLatkolik SLfalseC13 Megastigmane Derivatives From <i>Epipremnum pinnatum:</i> β-Damascenone Inhibits the Expression of Pro-Inflammatory Cytokines and Leukocyte Adhesion Molecules as Well as NF-κB Signaling.In order to identify active constituents and to gain some information regarding their mode of action, extracts from leaves of <i>Epipremnum pinnatum</i> were tested for their ability to inhibit inflammatory gene expression in endothelial- and monocyte-like cells (HUVECtert and THP-1, respectively). Bioactivity-guided fractionation using expression of <i>PTGS2</i> (COX-2) mRNA as a readout resulted in the isolation of two C13 megastigmane glycosides, gusanlungionoside C (<b>1</b>) and citroside A (<b>3</b>), and the phenylalcohol glycoside phenylmethyl-2-<i>O</i>-(6-<i>O</i>-rhamnosyl)-ß-D-galactopyranoside (<b>2</b>). Further analysis identified six additional megastigmane glycosides and the aglycones β-damascenone (<b>10</b>), megastigmatrienone (<b>11</b>), 3-hydroxy-β-damascenone (<b>122019-01-01T00:00:00Z20192025-04-25T21:08:02.105Z2020-05-22T00:01:40ZS-EPMC68929673184964110.3389/fphar.2019.01351