biostudies-literatureUnknown33(12)Graves JPInflammatory stimuli, such as bacterial LPS, alter the expression of many cytochromes P450. CYP2C and CYP2J subfamily members actively metabolize fatty acids to bioactive eicosanoids, which exhibit potent anti-inflammatory effects. Herein, we examined mRNA levels of the 15 mouse <i>Cyp2c</i> and 7 mouse <i>Cyp2j</i> isoforms in liver, kidney, duodenum, and brain over a 96-h time course of LPS-induced inflammation and resolution. Plasma and liver eicosanoid levels were also measured by liquid chromatography with tandem mass spectrometry. Expression changes in <i>Cyp2c</i> and <i>Cyp2j</i> isoforms were both isoform and tissue specific. Total liver <i>Cyp2c</i> and <i>Cyp2j</i> mRNA content was reduced by 80% 24 h after LPS but recovered to baseline levels by 96 h. Total <i>Cyp2c</i> and <i>FASEB journal : official publication of the Federation of American Societies for Experimental Biology14784-14797https://www.ebi.ac.uk/biostudies/studies/S-EPMC6894073biostudies-literatureExpression of <i>Cyp2c</i>/<i>Cyp2j</i> subfamily members and oxylipin levels during LPS-induced inflammation and resolution in mice.PMC6894073Li HZeldin DCGraves JPLih FBEdin MLDuval CBradbury JAGruzdev AfalseExpression of <i>Cyp2c</i>/<i>Cyp2j</i> subfamily members and oxylipin levels during LPS-induced inflammation and resolution in mice.Inflammatory stimuli, such as bacterial LPS, alter the expression of many cytochromes P450. CYP2C and CYP2J subfamily members actively metabolize fatty acids to bioactive eicosanoids, which exhibit potent anti-inflammatory effects. Herein, we examined mRNA levels of the 15 mouse <i>Cyp2c</i> and 7 mouse <i>Cyp2j</i> isoforms in liver, kidney, duodenum, and brain over a 96-h time course of LPS-induced inflammation and resolution. Plasma and liver eicosanoid levels were also measured by liquid chromatography with tandem mass spectrometry. Expression changes in <i>Cyp2c</i> and <i>Cyp2j</i> isoforms were both isoform and tissue specific. Total liver <i>Cyp2c</i> and <i>Cyp2j</i> mRNA content was reduced by 80% 24 h after LPS but recovered to baseline levels by 96 h. Total <i>Cyp2c</i> and <i>2019-01-01T00:00:00Z2019 Dec2025-05-18T11:51:10.958Z2025-05-18T11:51:10.958ZS-EPMC68940733169012510.1096/fj.201901872R10.1096/fj.201901872r