biostudies-literatureMaximo HJCNPqFapesp544https://www.ebi.ac.uk/biostudies/studies/S-EPMC6896422biostudies-literatureUnknown19(1)<h4>Background</h4>Phytophthora species secrete cytoplasmic effectors from a family named Crinkler (CRN), which are characterised by the presence of conserved specific domains in the N- and C-terminal regions. P. parasitica causes disease in a wide range of host plants, however the role of CRN effectors in these interactions remains unclear. Here, we aimed to: (i) identify candidate CRN encoding genes in P. parasitica genomes; (ii) evaluate the transcriptional expression of PpCRN (Phytophthora parasitica Crinkler candidate) during the P. parasitica interaction with Citrus sunki (high susceptible) and Poncirus trifoliata (resistant); and (iii) functionally characterize two PpCRNs in the model plant Nicotiana benthamiana.<h4>Results</h4>Our in silico analyses identified 80 putative PpCRN effBMC plant biologyPpCRN7 and PpCRN20 of Phythophthora parasitica regulate plant cell death leading to enhancement of host susceptibility.PMC68964222015/14498-6465450/2014-22014/50880-4Felizatti HLDias RODalio RJDMaximo HJMachado MALitholdo CGfalsePpCRN7 and PpCRN20 of Phythophthora parasitica regulate plant cell death leading to enhancement of host susceptibility.<h4>Background</h4>Phytophthora species secrete cytoplasmic effectors from a family named Crinkler (CRN), which are characterised by the presence of conserved specific domains in the N- and C-terminal regions. P. parasitica causes disease in a wide range of host plants, however the role of CRN effectors in these interactions remains unclear. Here, we aimed to: (i) identify candidate CRN encoding genes in P. parasitica genomes; (ii) evaluate the transcriptional expression of PpCRN (Phytophthora parasitica Crinkler candidate) during the P. parasitica interaction with Citrus sunki (high susceptible) and Poncirus trifoliata (resistant); and (iii) functionally characterize two PpCRNs in the model plant Nicotiana benthamiana.<h4>Results</h4>Our in silico analyses identified 80 putative PpCRN eff2019-01-01T00:00:00Z2019 Dec2026-05-03T13:10:23.265Z2020-05-21T22:25:38ZS-EPMC68964223181045110.1186/s12870-019-2129-8