biostudies-literatureUnknown57(3)Suttisuwan RThis study aims to determine the antioxidant activity of bioactive peptides derived from <i>Synechococcus</i> sp. VDW cells cultured for 21 days. <i>Synechococcus</i> sp. VDW protein hydrolysates were prepared with trypsin and purified by ultrafiltration with molecular mass cut-off membranes of 10, 5 and 3 kDa. The <i>M</i><3 kDa (F_A) fraction had the highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with IC₅₀ values of (11.5±0.3) and (13.6±0.2) µg/mL, respectively. The F_A fraction was separated by reversed phase HPLC to yield four subfractions (F_1-4). The F₄ subfraction showed the highest maximum ABTS radical scavenging activity (3.55±0.61) %Food technology and biotechnology358-368https://www.ebi.ac.uk/biostudies/studies/S-EPMC6902293biostudies-literatureFree Radical Scavenging Properties and Induction of Apoptotic Effects of Fa Fraction Obtained after Proteolysis of Bioactive Peptides from Microalgae <i>Synechococcus</i> sp. VDW.PMC6902293Thongchul NKarnchanatat ASuttisuwan RSaisavoey TPhunpruch SSangtanoo PfalseFree Radical Scavenging Properties and Induction of Apoptotic Effects of Fa Fraction Obtained after Proteolysis of Bioactive Peptides from Microalgae <i>Synechococcus</i> sp. VDW.This study aims to determine the antioxidant activity of bioactive peptides derived from <i>Synechococcus</i> sp. VDW cells cultured for 21 days. <i>Synechococcus</i> sp. VDW protein hydrolysates were prepared with trypsin and purified by ultrafiltration with molecular mass cut-off membranes of 10, 5 and 3 kDa. The <i>M</i><3 kDa (F_A) fraction had the highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with IC₅₀ values of (11.5±0.3) and (13.6±0.2) µg/mL, respectively. The F_A fraction was separated by reversed phase HPLC to yield four subfractions (F_1-4). The F₄ subfraction showed the highest maximum ABTS radical scavenging activity (3.55±0.61) %2019-01-01T00:00:00Z2019 Sep2025-05-29T21:37:40.443Z2025-05-29T21:37:40.443ZS-EPMC69022933186674910.17113/ftb.57.03.19.6028