biostudies-literatureSonnenblick ANCI NIH HHS424-432https://www.ebi.ac.uk/biostudies/studies/S-EPMC6903891biostudies-literatureUnknown52(2)In the present study, in order to investigate the role of signal transducer and activator of transcription 3 (STAT3) in estrogen receptor (ER)-positive breast cancer prognosis, we evaluated the phosphorylated STAT3 (p-STAT3) status and investigated its effect on the outcome in a pooled analysis and in a large prospective adjuvant trial. By using the TCGA repository, we developed gene signatures that reflected the level of p-STAT3. Using pooled analysis of the expression data from luminal breast cancer patients, we assessed the effects of the p-STAT3 expression signature on prognosis. We further validated the p-STAT3 prognostic effect using immunohistochemistry (IHC) and immunofluorescence staining of p-STAT3 tissue microarrays from a large randomised prospective trial. Our analysis demonstInternational journal of oncologyp-STAT3 in luminal breast cancer: Integrated RNA-protein pooled analysis and results from the BIG 2-98 phase III trial.PMC6903891P30 CA008748Viale GDe Azambuja EPeretz TCrown JPADaly LFrancis PASotiriou CJavdan BZahavi TDi Leo AFujisawa SLieveke APiccart MJSalmon ARouas GSonnenblick ASalgado RBrohee SBromberg JFVan den Eynden Gfalsep-STAT3 in luminal breast cancer: Integrated RNA-protein pooled analysis and results from the BIG 2-98 phase III trial.In the present study, in order to investigate the role of signal transducer and activator of transcription 3 (STAT3) in estrogen receptor (ER)-positive breast cancer prognosis, we evaluated the phosphorylated STAT3 (p-STAT3) status and investigated its effect on the outcome in a pooled analysis and in a large prospective adjuvant trial. By using the TCGA repository, we developed gene signatures that reflected the level of p-STAT3. Using pooled analysis of the expression data from luminal breast cancer patients, we assessed the effects of the p-STAT3 expression signature on prognosis. We further validated the p-STAT3 prognostic effect using immunohistochemistry (IHC) and immunofluorescence staining of p-STAT3 tissue microarrays from a large randomised prospective trial. Our analysis demonst2018-01-01T00:00:00Z2018 Feb2025-04-19T19:30:01.237Z2020-05-21T22:32:03ZS-EPMC69038912920708710.3892/ijo.2017.4212