{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["50(4)"],"submitter":["Nuaima RH"],"pubmed_abstract":["Populations of beet cyst nematodes <i>Heterodera schachtii</i> vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene <i>vap1</i> is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of <i>H. schachtii</i> based on the gene <i>vap1</i>. Degenerate primers were designed to amplify, clone, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for <i>H. schachtii</i>, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene vari"],"journal":["Journal of nematology"],"pagination":["517-528"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6909312"],"repository":["biostudies-literature"],"pubmed_title":["Effector gene <i>vap1</i> based DGGE fingerprinting to assess variation within and among <i>Heterodera schachtii</i> populations."],"pmcid":["PMC6909312"],"pubmed_authors":["Daub M","Hallmann J","Heuer H","Nuaima RH","Roeb J","Otte S"],"additional_accession":[]},"is_claimable":false,"name":"Effector gene <i>vap1</i> based DGGE fingerprinting to assess variation within and among <i>Heterodera schachtii</i> populations.","description":"Populations of beet cyst nematodes <i>Heterodera schachtii</i> vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene <i>vap1</i> is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of <i>H. schachtii</i> based on the gene <i>vap1</i>. Degenerate primers were designed to amplify, clone, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for <i>H. schachtii</i>, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene vari","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-18T16:37:52.541Z","creation":"2020-05-22T08:43:09Z"},"accession":"S-EPMC6909312","cross_references":{"pubmed":["31094153"],"doi":["10.21307/jofnem-2018-055"]}}