biostudies-literatureUnknown50(4)Nuaima RHPopulations of beet cyst nematodes <i>Heterodera schachtii</i> vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene <i>vap1</i> is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of <i>H. schachtii</i> based on the gene <i>vap1</i>. Degenerate primers were designed to amplify, clone, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for <i>H. schachtii</i>, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene variJournal of nematology517-528https://www.ebi.ac.uk/biostudies/studies/S-EPMC6909312biostudies-literatureEffector gene <i>vap1</i> based DGGE fingerprinting to assess variation within and among <i>Heterodera schachtii</i> populations.PMC6909312Daub MHallmann JHeuer HNuaima RHRoeb JOtte SfalseEffector gene <i>vap1</i> based DGGE fingerprinting to assess variation within and among <i>Heterodera schachtii</i> populations.Populations of beet cyst nematodes <i>Heterodera schachtii</i> vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene <i>vap1</i> is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of <i>H. schachtii</i> based on the gene <i>vap1</i>. Degenerate primers were designed to amplify, clone, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for <i>H. schachtii</i>, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene vari2018-01-01T00:00:00Z20182025-04-18T16:37:52.541Z2020-05-22T08:43:09ZS-EPMC69093123109415310.21307/jofnem-2018-055