biostudies-literatureWu SSichuan Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of China433https://www.ebi.ac.uk/biostudies/studies/S-EPMC6909630biostudies-literatureUnknown14(1)<h4>Objectives</h4>Methicillin-resistant Staphylococcus aureus (MRSA) strains present an urgent medical problem in osteomyelitis cases. Our previous study indicated that the YycFG two-component regulatory pathway is associated with the bacterial biofilm organization of MRSA strains. The aim of this study was to investigate the regulatory roles of ASyycG in the bacterial biofilm formation and the pathogenicity of MRSA strains using an antisense RNA strategy.<h4>Methods</h4>An ASyycG-overexpressing MRSA clinical isolate was constructed. The bacterial growth was monitored, and the biofilm biomass on bone specimens was examined using scanning electron microscopy and confocal laser scanning microscopy. Furthermore, quantitative RT-PCR (QRT-PCR) analysis was used to measure the expression of yycJournal of orthopaedic surgery and researchVirulence of methicillin-resistant Staphylococcus aureus modulated by the YycFG two-component pathway in a rat model of osteomyelitis.PMC69096302018SZ0125818009642019YFS0270Liu YLei LZhang HWu SfalseVirulence of methicillin-resistant Staphylococcus aureus modulated by the YycFG two-component pathway in a rat model of osteomyelitis.<h4>Objectives</h4>Methicillin-resistant Staphylococcus aureus (MRSA) strains present an urgent medical problem in osteomyelitis cases. Our previous study indicated that the YycFG two-component regulatory pathway is associated with the bacterial biofilm organization of MRSA strains. The aim of this study was to investigate the regulatory roles of ASyycG in the bacterial biofilm formation and the pathogenicity of MRSA strains using an antisense RNA strategy.<h4>Methods</h4>An ASyycG-overexpressing MRSA clinical isolate was constructed. The bacterial growth was monitored, and the biofilm biomass on bone specimens was examined using scanning electron microscopy and confocal laser scanning microscopy. Furthermore, quantitative RT-PCR (QRT-PCR) analysis was used to measure the expression of yyc2019-01-01T00:00:00Z2019 Dec2026-05-07T06:12:16.119Z2020-05-22T01:02:27ZS-EPMC69096303183103510.1186/s13018-019-1508-z