biostudies-literatureSchmitt KDeutsche ForschungsgemeinschaftE1384https://www.ebi.ac.uk/biostudies/studies/S-EPMC6912217biostudies-literatureUnknown8(11)Diverse signals and stress factors regulate the activity and homeostasis of ribosomes in all cells. The <i>Saccharomyces cerevisiae</i> protein Asc1/yRACK1 occupies an exposed site at the head region of the 40S ribosomal subunit (<i>hr40S</i>) and represents a central hub for signaling pathways. Asc1 strongly affects protein phosphorylation and is involved in quality control pathways induced by translation elongation arrest. Therefore, it is important to understand the dynamics of protein formations in the Asc1 microenvironment at the <i>hr40S</i>. We made use of the in vivo protein-proximity labeling technique Biotin IDentification (BioID). Unbiased proxiOMICs from two adjacent perspectives identified nucleocytoplasmic shuttling mRNA-binding proteins, the deubiquitinase complex Ubp3-Bre5,CellsyRACK1/Asc1 proxiOMICs-Towards Illuminating Ships Passing in the Night.PMC6912217VA 352/2-2A 630 (ZUK41/1)INST 186/1230-1 FUGGSchmitt KValerius OfalseyRACK1/Asc1 proxiOMICs-Towards Illuminating Ships Passing in the Night.Diverse signals and stress factors regulate the activity and homeostasis of ribosomes in all cells. The <i>Saccharomyces cerevisiae</i> protein Asc1/yRACK1 occupies an exposed site at the head region of the 40S ribosomal subunit (<i>hr40S</i>) and represents a central hub for signaling pathways. Asc1 strongly affects protein phosphorylation and is involved in quality control pathways induced by translation elongation arrest. Therefore, it is important to understand the dynamics of protein formations in the Asc1 microenvironment at the <i>hr40S</i>. We made use of the in vivo protein-proximity labeling technique Biotin IDentification (BioID). Unbiased proxiOMICs from two adjacent perspectives identified nucleocytoplasmic shuttling mRNA-binding proteins, the deubiquitinase complex Ubp3-Bre5,2019-01-01T00:00:00Z2019 Nov2026-04-08T03:02:15.943Z2020-05-22T07:11:36ZS-EPMC69122173168995510.3390/cells8111384