biostudies-literatureSchiller HBNHLBI NIH HHS1298-1310https://www.ebi.ac.uk/biostudies/studies/S-EPMC6913086biostudies-literatureUnknown196(10)<h4>Rationale</h4>Analyzing the molecular heterogeneity of different forms of organ fibrosis may reveal common and specific factors and thus identify potential future therapeutic targets.<h4>Objectives</h4>We sought to use proteome-wide profiling of human tissue fibrosis to (1) identify common and specific signatures across end-stage interstitial lung disease (ILD) cases, (2) characterize ILD subgroups in an unbiased fashion, and (3) identify common and specific features of lung and skin fibrosis.<h4>Methods</h4>We collected samples of ILD tissue (n = 45) and healthy donor control samples (n = 10), as well as fibrotic skin lesions from localized scleroderma and uninvolved skin (n = 6). Samples were profiled by quantitative label-free mass spectrometry, Western blotting, or confocal imagingAmerican journal of respiratory and critical care medicineDeep Proteome Profiling Reveals Common Prevalence of MZB1-Positive Plasma B Cells in Human Lung and Skin Fibrosis.PMC6913086R01 HL097163R33 HL120770Schwartz DAPreisendorfer SMoinzadeh PKrieg TStaab-Weijnitz CEckes BLeuschner GMann MHatz RABehr JSchiller HBMayr CHStrunz MEickelberg OfalseDeep Proteome Profiling Reveals Common Prevalence of MZB1-Positive Plasma B Cells in Human Lung and Skin Fibrosis.<h4>Rationale</h4>Analyzing the molecular heterogeneity of different forms of organ fibrosis may reveal common and specific factors and thus identify potential future therapeutic targets.<h4>Objectives</h4>We sought to use proteome-wide profiling of human tissue fibrosis to (1) identify common and specific signatures across end-stage interstitial lung disease (ILD) cases, (2) characterize ILD subgroups in an unbiased fashion, and (3) identify common and specific features of lung and skin fibrosis.<h4>Methods</h4>We collected samples of ILD tissue (n = 45) and healthy donor control samples (n = 10), as well as fibrotic skin lesions from localized scleroderma and uninvolved skin (n = 6). Samples were profiled by quantitative label-free mass spectrometry, Western blotting, or confocal imaging2017-01-01T00:00:00Z2017 Nov2025-05-29T22:03:33.617Z2025-05-29T22:03:33.617ZS-EPMC69130862865476410.1164/rccm.201611-2263OC10.1164/rccm.201611-2263oc