{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Carpenedo RL"],"funding":["Natural Sciences and Engineering Research Council of Canada","Canada Research Chair in Integrative Stem Cell Biology","Canadian Foundation for Innovation","Canadian Institutes of Health Research","CIHR"],"pagination":["1111-1125"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6915803"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(6)"],"pubmed_abstract":["Human pluripotent stem cells (hPSCs) are an essential cell source in tissue engineering, studies of development, and disease modeling. Efficient, broadly amenable protocols for rapid lineage induction of hPSCs are of great interest in the stem cell biology field. We describe a simple, robust method for differentiation of hPSCs into mesendoderm in defined conditions utilizing single-cell seeding (SCS) and BMP4 and Activin A (BA) treatment. BA treatment was readily incorporated into existing protocols for chondrogenic and endothelial progenitor cell differentiation, while fine-tuning of BA conditions facilitated definitive endoderm commitment. After prolonged differentiation in vitro or in vivo, BA pretreatment resulted in higher mesoderm and endoderm levels at the expense of ectoderm format"],"journal":["Stem cell reports"],"pubmed_title":["Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme."],"pmcid":["PMC6915803"],"funding_grant_id":["RGPIN 293170-11","MOP-89910","2016-06081"],"pubmed_authors":["Carpenedo RL","Choey C","Perkins TJ","Ho M","Stewart DJ","Kwon SY","Dore C","Stanford WL","Yockell-Lelievre J","Tanner RM"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme.","description":"Human pluripotent stem cells (hPSCs) are an essential cell source in tissue engineering, studies of development, and disease modeling. Efficient, broadly amenable protocols for rapid lineage induction of hPSCs are of great interest in the stem cell biology field. We describe a simple, robust method for differentiation of hPSCs into mesendoderm in defined conditions utilizing single-cell seeding (SCS) and BMP4 and Activin A (BA) treatment. BA treatment was readily incorporated into existing protocols for chondrogenic and endothelial progenitor cell differentiation, while fine-tuning of BA conditions facilitated definitive endoderm commitment. After prolonged differentiation in vitro or in vivo, BA pretreatment resulted in higher mesoderm and endoderm levels at the expense of ectoderm format","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2026-05-01T09:32:02.442Z","creation":"2025-05-29T22:05:50.4Z"},"accession":"S-EPMC6915803","cross_references":{"pubmed":["31813826"],"doi":["10.1016/j.stemcr.2019.11.001"]}}