biostudies-literatureSheen MRNCI NIH HHSLaura and John Arnold Foundatione45120https://www.ebi.ac.uk/biostudies/studies/S-EPMC6917490biostudies-literatureUnknown8As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Fiering et al., 2015) that described how we intended to replicate selected experiments from the paper 'Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis' (Goetz et al., 2011). Here we report the results. Primary mouse embryonic fibroblasts (pMEFs) expressing caveolin 1 (Cav1WT) demonstrated increased extracellular matrix remodeling <i>in vitro</i> compared to Cav1 deficient (Cav1KO) pMEFs, similar to the original study (Goetz et al., 2011). <i>In vivo</i>, we found higher levels of intratumoral stroma remodeling, determined by fibronectin fiber orientation, in tumors from cancer cells co-injected with Cav1WT pMEFs compared to cancer cells only oeLifeReplication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis.PMC6917490P30 CA023108Reproducibility Project: Cancer BiologyErrington TMFields JLPerfito NTsui RSheen MRNorthan BDenis AAng LHIorns ELacoste JFiering SfalseReplication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis.As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Fiering et al., 2015) that described how we intended to replicate selected experiments from the paper 'Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis' (Goetz et al., 2011). Here we report the results. Primary mouse embryonic fibroblasts (pMEFs) expressing caveolin 1 (Cav1WT) demonstrated increased extracellular matrix remodeling <i>in vitro</i> compared to Cav1 deficient (Cav1KO) pMEFs, similar to the original study (Goetz et al., 2011). <i>In vivo</i>, we found higher levels of intratumoral stroma remodeling, determined by fibronectin fiber orientation, in tumors from cancer cells co-injected with Cav1WT pMEFs compared to cancer cells only o2019-01-01T00:00:00Z2019 Dec2026-04-14T21:08:31.089Z2020-05-22T00:05:02ZS-EPMC69174903184564710.7554/eLife.45120