{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8"],"submitter":["Gruntman E"],"funding":["Howard Hughes Medical Institute"],"pubmed_abstract":["In flies, the direction of moving ON and OFF features is computed separately. T4 (ON) and T5 (OFF) are the first neurons in their respective pathways to extract a directionally selective response from their non-selective inputs. Our recent study of T4 found that the integration of offset depolarizing and hyperpolarizing inputs is critical for the generation of directional selectivity. However, T5s lack small-field inhibitory inputs, suggesting they may use a different mechanism. Here we used whole-cell recordings of T5 neurons and found a similar receptive field structure: fast depolarization and persistent, spatially offset hyperpolarization. By assaying pairwise interactions of local stimulation across the receptive field, we found no amplifying responses, only suppressive responses to the non-preferred motion direction. We then evaluated passive, biophysical models and found that a model using direct inhibition, but not the removal of excitation, can accurately predict T5 responses to a range of moving stimuli."],"journal":["eLife"],"pagination":["e50706"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6917495"],"repository":["biostudies-literature"],"pubmed_title":["The computation of directional selectivity in the <i>Drosophila</i> OFF motion pathway."],"pmcid":["PMC6917495"],"pubmed_authors":["Gruntman E","Romani S","Reiser MB"],"additional_accession":[]},"is_claimable":false,"name":"The computation of directional selectivity in the <i>Drosophila</i> OFF motion pathway.","description":"In flies, the direction of moving ON and OFF features is computed separately. T4 (ON) and T5 (OFF) are the first neurons in their respective pathways to extract a directionally selective response from their non-selective inputs. Our recent study of T4 found that the integration of offset depolarizing and hyperpolarizing inputs is critical for the generation of directional selectivity. However, T5s lack small-field inhibitory inputs, suggesting they may use a different mechanism. Here we used whole-cell recordings of T5 neurons and found a similar receptive field structure: fast depolarization and persistent, spatially offset hyperpolarization. By assaying pairwise interactions of local stimulation across the receptive field, we found no amplifying responses, only suppressive responses to the non-preferred motion direction. We then evaluated passive, biophysical models and found that a model using direct inhibition, but not the removal of excitation, can accurately predict T5 responses to a range of moving stimuli.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2024-02-16T04:15:20.715Z","creation":"2020-05-21T23:36:36Z"},"accession":"S-EPMC6917495","cross_references":{"pubmed":["31825313"],"doi":["10.7554/eLife.50706"]}}