{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Srivastav SP"],"funding":["NICHD NIH HHS","NIA NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["e49948"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6917496"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8"],"pubmed_abstract":["Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in <i>Drosophila</i> called gonadal dysgenesis (GD). <i>Harwich</i> (<i>Har</i>) strains with <i>P</i>-elements cause severe GD in F1 daughters when <i>Har</i> fathers mate with mothers lacking <i>P</i>-element-piRNAs (i.e. <i>ISO1</i> strain). To address the mystery of why <i>Har</i> induces severe GD, we bred hybrid <i>Drosophila</i> with <i>Har</i> genomic fragments into the <i>ISO1</i> background to create <i>HISR-D or HISR-N</i> lines that still cause <u>D</u>ysgenesis or are <u>N</u>on-dysgenic, respectively. In these lines, we discovered a highly truncated <i>P</i>-element variant we named '<i>Har-P</i>' as the most frequent de novo insertion. Although <i>HISR-D</i> lines st"],"journal":["eLife"],"pubmed_title":["<i>Har-P</i>, a short <i>P</i>-element variant, weaponizes <i>P</i>-transposase to severely impair <i>Drosophila</i> development."],"pmcid":["PMC6917496"],"funding_grant_id":["R21-HD088792","R01-AG052465","R21 HD088792","R01 AG052465"],"pubmed_authors":["Rahman R","Ma Q","Pierre J","Bandyopadhyay S","Lau NC","Srivastav SP"],"additional_accession":[]},"is_claimable":false,"name":"<i>Har-P</i>, a short <i>P</i>-element variant, weaponizes <i>P</i>-transposase to severely impair <i>Drosophila</i> development.","description":"Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in <i>Drosophila</i> called gonadal dysgenesis (GD). <i>Harwich</i> (<i>Har</i>) strains with <i>P</i>-elements cause severe GD in F1 daughters when <i>Har</i> fathers mate with mothers lacking <i>P</i>-element-piRNAs (i.e. <i>ISO1</i> strain). To address the mystery of why <i>Har</i> induces severe GD, we bred hybrid <i>Drosophila</i> with <i>Har</i> genomic fragments into the <i>ISO1</i> background to create <i>HISR-D or HISR-N</i> lines that still cause <u>D</u>ysgenesis or are <u>N</u>on-dysgenic, respectively. In these lines, we discovered a highly truncated <i>P</i>-element variant we named '<i>Har-P</i>' as the most frequent de novo insertion. Although <i>HISR-D</i> lines st","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2026-04-14T21:08:34.292Z","creation":"2020-05-22T00:05:07Z"},"accession":"S-EPMC6917496","cross_references":{"pubmed":["31845649"],"doi":["10.7554/eLife.49948"]}}