{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Njouendou AJ"],"funding":["Bundesministerium für Bildung und Forschung","National Centre for the Replacement, Refinement and Reduction of Animals in Research","Deutsche Forschungsgemeinschaft"],"pagination":["104930"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6920388"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28"],"pubmed_abstract":["Endemic communities of <i>Mansonella perstans</i> infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy have also been ignored despite a large number of infected individuals in areas of Cameroon. Thus, we established an <i>in vitro</i> system to culture <i>M. perstans</i> microfilariae (Mf); the transmission stage of infection. In short, we compared the ability of two renowned culture media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) to sustain Mf in culture. Media were supplemented with 10% fetal bovine serum (FBS) and monkey kidney epithelial cells (LLC-MK<sub>2</sub>) were used as feeder cells. As readout we assessed Mf survival and motility using a standardised microscopy assessment strategy. Moreover, this <i>in vitro</i> culture system was used to test susceptibility levels of microfilariae to different chemotherapeutic agents. Parasite motility was scored daily using a graded system and analysed using the average motility and area under the motility curve of <i>M. perstans</i> Mf. These datasets were analysed and discussed in detail in the related article entitled: \"<i>In vitro</i> maintenance of <i>Mansonella perstans</i> microfilariae and its relevance for drug screening\" [1]."],"journal":["Data in brief"],"pubmed_title":["Dataset on &lt;i&gt;in vitro&lt;/i&gt; maintenance of &lt;i&gt;Mansonella perstans&lt;/i&gt; microfilariae and drug testing."],"pmcid":["PMC6920388"],"funding_grant_id":["NC/M00175X/1","HO 2009/14-1","DFG HO2009/10-1"],"pubmed_authors":["Fombad FF","Enyong P","Njouendou AJ","Ritter M","Ndongmo WPC","Layland LE","Gandjui NVT","Hoerauf A","Wanji S","Njiokou F","Turner J","Kien CA","Pfarr K","Esum ME"],"additional_accession":[]},"is_claimable":false,"name":"Dataset on &lt;i&gt;in vitro&lt;/i&gt; maintenance of &lt;i&gt;Mansonella perstans&lt;/i&gt; microfilariae and drug testing.","description":"Endemic communities of <i>Mansonella perstans</i> infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy have also been ignored despite a large number of infected individuals in areas of Cameroon. Thus, we established an <i>in vitro</i> system to culture <i>M. perstans</i> microfilariae (Mf); the transmission stage of infection. In short, we compared the ability of two renowned culture media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) to sustain Mf in culture. Media were supplemented with 10% fetal bovine serum (FBS) and monkey kidney epithelial cells (LLC-MK<sub>2</sub>) were used as feeder cells. As readout we assessed Mf survival and motility using a standardised microscopy assessment strategy. Moreover, this <i>in vitro</i> culture system was used to test susceptibility levels of microfilariae to different chemotherapeutic agents. Parasite motility was scored daily using a graded system and analysed using the average motility and area under the motility curve of <i>M. perstans</i> Mf. These datasets were analysed and discussed in detail in the related article entitled: \"<i>In vitro</i> maintenance of <i>Mansonella perstans</i> microfilariae and its relevance for drug screening\" [1].","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Feb","modification":"2026-06-12T03:18:41.697Z","creation":"2020-05-22T01:11:30Z"},"accession":"S-EPMC6920388","cross_references":{"pubmed":["31886361"],"doi":["10.1016/j.dib.2019.104930"]}}